Browsing by Author "de Souza, Robson F."
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- Global Distribution and Evolution of Mycobacterium bovis LineagesZimpel, Cristina Kraemer; Patane, Jose Salvatore L.; Proenga Guedes, Aureliano Coelho; de Souza, Robson F.; Silva-Pereira, Taiana T.; Soler Camargo, Naila C.; de Souza Filho, Antonio F.; Ikuta, Cassia Y.; Ferreira Neto, Jose Soares; Setubal, Joao Carlos; Heinemann, Marcos Bryan; Sa Guimaraes, Ana Marcia (2020-05-07)Mycobacterium bovis is the main causative agent of zoonotic tuberculosis in humans and frequently devastates livestock and wildlife worldwide. Previous studies suggested the existence of genetic groups of M. bovis strains based on limited DNA markers (a.k.a. clonal complexes), and the evolution and ecology of this pathogen has been only marginally explored at the global level. We have screened over 2,600 publicly available M. bovis genomes and newly sequenced four wildlife M. bovis strains, gathering 1,969 genomes from 23 countries and at least 24 host species, including humans, to complete a phylogenomic analyses. We propose the existence of four distinct global lineages of M. bovis (Lb1, Lb2, Lb3, and Lb4) underlying the current disease distribution. These lineages are not fully represented by clonal complexes and are dispersed based on geographic location rather than host species. Our data divergence analysis agreed with previous studies reporting independent archeological data of ancient M. bovis (South Siberian infected skeletons at similar to 2,000 years before present) and indicates that extant M. bovis originated between 715 and 3,556 years BP, with later emergence in the New World and Oceania, likely influenced by trades among countries.
- Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifoliiMoreira, Leandro M.; Almeida, Nalvo F.; Potnis, Neha; Digiampietri, Luciano A.; Adi, Said S.; Bortolossi, Julio C.; da Silva, Ana C.; da Silva, Aline M.; de Moraes, Fabrício E.; de Oliveira, Julio C.; de Souza, Robson F.; Facincani, Agda P.; Ferraz, André L.; Ferro, Maria I.; Furlan, Luiz R.; Gimenez, Daniele F.; Jones, Jeffrey B.; Kitajima, Elliot W.; Laia, Marcelo L.; Leite, Rui P., Jr; Nishiyama, Milton Y.; Rodrigues Neto, Julio; Nociti, Letícia A.; Norman, David J.; Ostroski, Eric H.; Pereira, Haroldo A. Jr.; Staskawicz, Brian J.; Tezza, Renata I.; Ferro, Jesus A.; Vinatzer, Boris A.; Setubal, João C. (Biomed Central, 2010-04-13)Background Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The three types have different phenotypes and affect different citrus species. The causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C. Results We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. In addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein. Conclusion We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. The gained knowledge will be instrumental for improving citrus canker control.