Fralin Life Sciences Institute

Permanent URI for this community

https://hdl.handle.net/10919/25796
Note: In 2019, the Biocomplexity Institute became part of the Fralin Life Sciences Institute.

Browse

Browsing Fralin Life Sciences Institute by Department "Civil and Environmental Engineering"

Now showing 1 - 7 of 7
  • Thumbnail Image
    Addressing the Contribution of Indirect Potable Reuse to Inland Freshwater Salinization
    Bhide, Shantanu V.; Grant, Stanley B.; Parker, Emily A.; Rippy, Megan A.; Godrej, Adil N.; Kaushal, Sujay S.; Prelewicz, Gregory; Saji, Niffy; Curtis, Shannon; Vikesland, Peter J.; Maile-Moskowitz, Ayella; Edwards, Marc A.; Lopez, Kathryn; Birkland, Thomas A.; Schenk, Todd (2021-02-02)
    Inland freshwater salinity is rising worldwide, a phenomenon called the freshwater salinization syndrome (FSS). We investigate a potential conflict between managing the FSS and indirect potable reuse, the practice of augmenting water supplies through the addition of reclaimed wastewater to surface waters and groundwaters. From time-series data collected over 25 years, we quantify the contributions of three salinity sources—a wastewater reclamation facility and two rapidly urbanizing watersheds—to the rising concentration of sodium (a major ion associated with the FSS) in a regionally important drinking water reservoir in the Mid-Atlantic United States. Sodium mass loading to the reservoir is primarily from watershed runoff during wet weather and reclaimed wastewater during dry weather. Across all timescales evaluated, sodium concentration in the reclaimed wastewater is higher than in outflow from the two watersheds. Sodium in reclaimed wastewater originates from chemicals added during wastewater treatment, industrial and commercial discharges, human excretion, and down-drain disposal of drinking water and sodium-rich household products. Thus, numerous opportunities exist to reduce the contribution of indirect potable reuse to sodium pollution at this site, and the FSS more generally. These efforts will require deliberative engagement with a diverse community of watershed stakeholders and careful consideration of the local political, social, and environmental context.
  • Thumbnail Image
    A One-Dimensional Model for Turbulent Mixing in the Benthic Biolayer of Stream and Coastal Sediments
    Grant, Stanley B.; Gomez-Velez, Jesus D.; Ghisalberti, Marco; Guymer, Ian; Boano, Fulvio; Roche, Kevin; Harvey, Judson (2020-12)
    In this paper, we develop and validate a rigorous modeling framework, based on Duhamel's Theorem, for the unsteady one-dimensional vertical transport of a solute across a flat sediment-water interface (SWI) and through the benthic biolayer of a turbulent stream. The modeling framework is novel in capturing the two-way coupling between evolving solute concentrations above and below the SWI and in allowing for a depth-varying diffusivity. Three diffusivity profiles within the sediment (constant, exponentially decaying, and a hybrid model) are evaluated against an extensive set of previously published laboratory measurements of turbulent mass transfer across the SWI. The exponential diffusivity profile best represents experimental observations and its reference diffusivity scales with the permeability Reynolds number, a dimensionless measure of turbulence at the SWI. The depth over which turbulence-enhanced diffusivity decays is of the order of centimeters and comparable to the thickness of the benthic biolayer. Thus, turbulent mixing across the SWI may serve as a universal transport mechanism, supplying the nutrient and energy fluxes needed to sustain microbial growth, and nutrient processing, in the benthic biolayer of stream and coastal sediments.
  • Thumbnail Image
    Predicting Solute Transport Through Green Stormwater Infrastructure With Unsteady Transit Time Distribution Theory
    Parker, E. A.; Grant, Stanley B.; Cao, Y.; Rippy, Megan A.; McGuire, Kevin J.; Holden, P. A.; Feraud, M.; Avasarala, S.; Liu, H.; Hung, W. C.; Rugh, M.; Jay, J.; Peng, J.; Shao, S.; Li, D. (2021-02)
    In this study, we explore the use of unsteady transit time distribution (TTD) theory to model solute transport in biofilters, a popular form of nature-based or "green" storm water infrastructure (GSI). TTD theory has the potential to address many unresolved challenges associated with predicting pollutant fate and transport through these systems, including unsteadiness in the water balance (time-varying inflows, outflows, and storage), unsteadiness in pollutant loading, time-dependent reactions, and scale-up to GSI networks and urban catchments. From a solution to the unsteady age conservation equation under uniform sampling, we derive an explicit expression for solute breakthrough during and after one or more storm events. The solution is calibrated and validated with breakthrough data from 17 simulated storms at a field-scale biofilter test facility in Southern California, using bromide as a conservative tracer. TTD theory closely reproduces bromide breakthrough concentrations, provided that lateral exchange with the surrounding soil is accounted for. At any given time, according to theory, more than half of the water in storage is from the most recent storm, while the rest is a mixture of penultimate and earlier storms. Thus, key management endpoints, such as the pollutant treatment credit attributable to GSI, are likely to depend on the evolving age distribution of water stored and released by these systems.
  • Thumbnail Image
    Probiotics and virulent human rotavirus modulate the transplanted human gut microbiota in gnotobiotic pigs
    Zhang, Husen; Wang, Haifeng; Shepherd, Megan L.; Wen, Ke; Li, Guohua; Yang, Xingdong; Kocher, Jacob; Giri-Rachman, Ernawati; Dickerman, Allan W.; Settlage, Robert E.; Yuan, Lijuan (2014-09-09)
    We generated a neonatal pig model with human infant gut microbiota (HGM) to study the effect of a probiotic on the composition of the transplanted microbiota following rotavirus vaccination and challenge. All the HGM-transplanted pigs received two doses of an oral attenuated rotavirus vaccine. The gut microbiota of vaccinated pigs were investigated for effects of Lactobacillus rhamnosus GG (LGG) supplement and homotypic virulent human rotavirus (HRV) challenge. High-throughput sequencing of V4 region of 16S rRNA genes demonstrated that HGM-transplanted pigs carried microbiota similar to that of the C-section delivered baby. Firmicutes and Proteobacteria represented over 98% of total bacteria in the human donor and the recipient pigs. HRV challenge caused a phylum-level shift from Firmicutes to Proteobacteria. LGG supplement prevented the changes in microbial communities caused by HRV challenge. In particular, members of Enterococcus in LGG-supplemented pigs were kept at the baseline level, while they were enriched in HRV challenged pigs. Taken together, our results suggested that HGM pigs are valuable for testing the microbiota’s response to probiotic interventions for treating infantile HRV infection.
  • Thumbnail Image
    Research Needs, Challenges, and Strategic Approaches for Natural Hazards and Disaster Reconnaissance
    Wartman, Joseph; Berman, Jeffrey W.; Bostrom, Ann; Miles, Scott B.; Olsen, Michael; Gurley, Kurtis; Irish, Jennifer L.; Lowes, Laura; Tanner, Troy; Dafni, Jake; Grilliot, Michael; Lyda, Andrew; Peltier, Jaqueline (2020-11-10)
    Natural hazards and disaster reconnaissance investigations have provided many lessons for the research and practice communities and have greatly improved our scientific understanding of extreme events. Yet, many challenges remain for these communities, including improving our ability to model hazards, make decisions in the face of uncertainty, enhance community resilience, and mitigate risk. State-of-the-art instrumentation and mobile data collection applications have significantly advanced the ability of field investigation teams to capture quickly perishable data in post-disaster settings. The NHERI RAPID Facility convened a community workshop of experts in the professional, government, and academic sectors to determine reconnaissance data needs and opportunities, and to identify the broader challenges facing the reconnaissance community that hinder data collection and use. Participants highlighted that field teams face many practical and operational challenges before and during reconnaissance investigations, including logistics concerns, safety issues, emotional trauma, and after-returning, issues with data processing and analysis. Field teams have executed many effective missions. Among the factors contributing to successful reconnaissance are having local contacts, effective teamwork, and pre-event training. Continued progress in natural hazard reconnaissance requires adaptation of new, strategic approaches that acquire and integrate data over a range of temporal, spatial, and social scales across disciplines.
  • Thumbnail Image
    Single-Cell Analysis Reveals that Chronic Silver Nanoparticle Exposure Induces Cell Division Defects in Human Epithelial Cells
    Garcia, Ellen B.; Alms, Cynthia; Hinman, Albert W.; Kelly, Conor; Smith, Adam; Vance, Marina; Loncarek, Jadranka; Marr, Linsey C.; Cimini, Daniela (MDPI, 2019-06-11)
    Multiple organizations have urged a paradigm shift from traditional, whole animal, chemical safety testing to alternative methods. Although these forward-looking methods exist for risk assessment and predication, animal testing is still the preferred method and will remain so until more robust cellular and computational methods are established. To meet this need, we aimed to develop a new, cell division-focused approach based on the idea that defective cell division may be a better predictor of risk than traditional measurements. To develop such an approach, we investigated the toxicity of silver nanoparticles (AgNPs) on human epithelial cells. AgNPs are the type of nanoparticle most widely employed in consumer and medical products, yet toxicity reports are still confounding. Cells were exposed to a range of AgNP doses for both short- and-long term exposure times. The analysis of treated cell populations identified an effect on cell division and the emergence of abnormal nuclear morphologies, including micronuclei and binucleated cells. Overall, our results indicate that AgNPs impair cell division, not only further confirming toxicity to human cells, but also highlighting the propagation of adverse phenotypes within the cell population. Furthermore, this work illustrates that cell division-based analysis will be an important addition to future toxicology studies.
  • Thumbnail Image
    Unifying Advective and Diffusive Descriptions of Bedform Pumping in the Benthic Biolayer of Streams
    Grant, Stanley B.; Monofy, Ahmed; Boano, Fulvio; Gomez-Velez, Jesus D.; Guymer, Ian; Harvey, Judson; Ghisalberti, Marco (2020-09-01)
    Many water quality and ecosystem functions performed by streams occur in the benthic biolayer, the biologically active upper (similar to 5 cm) layer of the streambed. Solute transport through the benthic biolayer is facilitated by bedform pumping, a physical process in which dynamic and static pressure variations over the surface of stationary bedforms (e.g., ripples and dunes) drive flow across the sediment-water interface. In this paper we derive two predictive modeling frameworks, one advective and the other diffusive, for solute transport through the benthic biolayer by bedform pumping. Both frameworks closely reproduce patterns and rates of bedform pumping previously measured in the laboratory, provided that the diffusion model's dispersion coefficient declines exponentially with depth. They are also functionally equivalent, such that parameter sets inferred from the 2D advective model can be applied to the 1D diffusive model, and vice versa. The functional equivalence and complementary strengths of these two models expand the range of questions that can be answered, for example, by adopting the 2D advective model to study the effects of geomorphic processes (such as bedform adjustments to land use change) on flow-dependent processes and the 1D diffusive model to study problems where multiple transport mechanisms combine (such as bedform pumping and turbulent diffusion). By unifying 2D advective and 1D diffusive descriptions of bedform pumping, our analytical results provide a straightforward and computationally efficient approach for predicting, and better understanding, solute transport in the benthic biolayer of streams and coastal sediments.