Virginia Tech
    • Log in
    View Item 
    •   VTechWorks Home
    • College of Science (COS)
    • Department of Biological Sciences
    • Scholarly Works, Department of Biological Sciences
    • View Item
    •   VTechWorks Home
    • College of Science (COS)
    • Department of Biological Sciences
    • Scholarly Works, Department of Biological Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease

    Thumbnail
    View/Open
    1747.full.pdf (392.8Kb)
    Downloads: 506
    Date
    2013-03-27
    Author
    Wallace, Richard J. Jr.
    Iakhiaeva, Elena
    Williams, Myra D.
    Brown-Elliott, Barbara A.
    Vasireddy, Sruthi
    Vasireddy, Ravikiran
    Lande, Leah
    Peterson, Donald D.
    Sawicki, Janet
    Kwait, Rebecca
    Tichenor, Wellington S.
    Turenne, Christine
    Falkinham, Joseph O. III
    Metadata
    Show full item record
    Abstract
    Recent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm isolates of Mycobacterium avium could be matched by DNA fingerprinting. To determine if this is true for Mycobacterium intracellulare, household water sources for 36 patients with Mycobacterium avium complex (MAC) lung disease were evaluated. MAC household water isolates from three published studies that included 37 additional MAC respiratory disease patients were also evaluated. Species identification was done initially using nonsequencing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene sequencing. M. intracellulare was identified by nonsequencing methods in 54 respiratory cultures and 41 household water/biofilm samples. By ITS sequencing, 49 (90.7%) respiratory isolates were M. intracellulare and 4 (7.4%) were Mycobacterium chimaera. In contrast, 30 (73%) household water samples were M. chimaera, 8 (20%) were other MAC X species (i.e., isolates positive with a MAC probe but negative with species-specific M. avium and M. intracellulare probes), and 3 (7%) were M. avium; none were M. intracellulare. In comparison, M. avium was recovered from 141 water/biofilm samples. These results indicate that M. intracellulare lung disease in the United States is acquired from environmental sources other than household water. Nonsequencing methods for identification of nontuberculous mycobacteria (including those of the MAC) might fail to distinguish closely related species (such as M. intracellulare and M. chimaera). This is the first report of M. chimaera recovery from household water. The study underscores the importance of taxonomy and distinguishing the many species and subspecies of the MAC.
    URI
    http://hdl.handle.net/10919/64225
    Collections
    • Scholarly Works, Department of Biological Sciences [875]

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us
     

     

    VTechWorks

    AboutPoliciesHelp

    Browse

    All of VTechWorksCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Log inRegister

    Statistics

    View Usage Statistics

    If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated.

    Virginia Tech | University Libraries | Contact Us