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dc.contributorVirginia Techen
dc.contributor.authorWallace, Richard J. Jr.en
dc.contributor.authorIakhiaeva, Elenaen
dc.contributor.authorWilliams, Myra D.en
dc.contributor.authorBrown-Elliott, Barbara A.en
dc.contributor.authorVasireddy, Sruthien
dc.contributor.authorVasireddy, Ravikiranen
dc.contributor.authorLande, Leahen
dc.contributor.authorPeterson, Donald D.en
dc.contributor.authorSawicki, Janeten
dc.contributor.authorKwait, Rebeccaen
dc.contributor.authorTichenor, Wellington S.en
dc.contributor.authorTurenne, Christineen
dc.contributor.authorFalkinham, Joseph O. IIIen
dc.date.accessioned2015-11-29T04:13:28Zen
dc.date.available2015-11-29T04:13:28Zen
dc.date.issued2013-03-27en
dc.identifier.citationWallace, Richard J., Jr. et al. (2013). Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease. Journal of Clinical Microbiology, 51(6), 1747-1752. doi:10.1128/jcm.00186-13en
dc.identifier.issn0095-1137en
dc.identifier.urihttp://hdl.handle.net/10919/64225en
dc.description.abstractRecent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm isolates of Mycobacterium avium could be matched by DNA fingerprinting. To determine if this is true for Mycobacterium intracellulare, household water sources for 36 patients with Mycobacterium avium complex (MAC) lung disease were evaluated. MAC household water isolates from three published studies that included 37 additional MAC respiratory disease patients were also evaluated. Species identification was done initially using nonsequencing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene sequencing. M. intracellulare was identified by nonsequencing methods in 54 respiratory cultures and 41 household water/biofilm samples. By ITS sequencing, 49 (90.7%) respiratory isolates were M. intracellulare and 4 (7.4%) were Mycobacterium chimaera. In contrast, 30 (73%) household water samples were M. chimaera, 8 (20%) were other MAC X species (i.e., isolates positive with a MAC probe but negative with species-specific M. avium and M. intracellulare probes), and 3 (7%) were M. avium; none were M. intracellulare. In comparison, M. avium was recovered from 141 water/biofilm samples. These results indicate that M. intracellulare lung disease in the United States is acquired from environmental sources other than household water. Nonsequencing methods for identification of nontuberculous mycobacteria (including those of the MAC) might fail to distinguish closely related species (such as M. intracellulare and M. chimaera). This is the first report of M. chimaera recovery from household water. The study underscores the importance of taxonomy and distinguishing the many species and subspecies of the MAC.en
dc.description.sponsorshipAmon G. Carter Foundationen
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherAmerican Society for Microbiologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleAbsence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Diseaseen
dc.typeArticle - Refereeden
dc.rights.holderAmerican Society for Microbiologyen
dc.contributor.departmentBiological Sciencesen
dc.identifier.urlhttp://jcm.asm.org/content/51/6/1747en
dc.date.accessed2015-11-28en
dc.title.serialJournal of Clinical Microbiologyen
dc.identifier.doihttps://doi.org/10.1128/jcm.00186-13en
dc.type.dcmitypeTexten


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