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dc.contributorVirginia Techen_US
dc.contributor.authorWallace, Richard J., Jr.en_US
dc.contributor.authorIakhiaeva, Elenaen_US
dc.contributor.authorWilliams, Myra D.en_US
dc.contributor.authorBrown-Elliott, Barbara A.en_US
dc.contributor.authorVasireddy, Sruthien_US
dc.contributor.authorVasireddy, Ravikiranen_US
dc.contributor.authorLande, Leahen_US
dc.contributor.authorPeterson, Donald D.en_US
dc.contributor.authorSawicki, Janeten_US
dc.contributor.authorKwait, Rebeccaen_US
dc.contributor.authorTichenor, Wellington S.en_US
dc.contributor.authorTurenne, Christineen_US
dc.contributor.authorFalkinham, Joseph O. IIIen_US
dc.date.accessioned2015-11-29T04:13:28Z
dc.date.available2015-11-29T04:13:28Z
dc.date.issued2013-03-27en_US
dc.identifier.citationWallace, Richard J., Jr. et al. (2013). Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease. Journal of Clinical Microbiology, 51(6), 1747-1752. doi:10.1128/jcm.00186-13en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10919/64225
dc.description.abstractRecent studies have shown that respiratory isolates from pulmonary disease patients and household water/biofilm isolates of Mycobacterium avium could be matched by DNA fingerprinting. To determine if this is true for Mycobacterium intracellulare, household water sources for 36 patients with Mycobacterium avium complex (MAC) lung disease were evaluated. MAC household water isolates from three published studies that included 37 additional MAC respiratory disease patients were also evaluated. Species identification was done initially using nonsequencing methods with confirmation by internal transcribed spacer (ITS) and/or partial 16S rRNA gene sequencing. M. intracellulare was identified by nonsequencing methods in 54 respiratory cultures and 41 household water/biofilm samples. By ITS sequencing, 49 (90.7%) respiratory isolates were M. intracellulare and 4 (7.4%) were Mycobacterium chimaera. In contrast, 30 (73%) household water samples were M. chimaera, 8 (20%) were other MAC X species (i.e., isolates positive with a MAC probe but negative with species-specific M. avium and M. intracellulare probes), and 3 (7%) were M. avium; none were M. intracellulare. In comparison, M. avium was recovered from 141 water/biofilm samples. These results indicate that M. intracellulare lung disease in the United States is acquired from environmental sources other than household water. Nonsequencing methods for identification of nontuberculous mycobacteria (including those of the MAC) might fail to distinguish closely related species (such as M. intracellulare and M. chimaera). This is the first report of M. chimaera recovery from household water. The study underscores the importance of taxonomy and distinguishing the many species and subspecies of the MAC.en_US
dc.description.sponsorshipAmon G. Carter Foundationen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsIn Copyright (InC)en_US
dc.rightsThis Item is protected by copyright and/or related rights. Some uses of this Item may be deemed fair and permitted by law even without permission from the rights holder(s). For other uses you need to obtain permission from the rights holder(s).en_US
dc.titleAbsence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Diseaseen_US
dc.typeArticle - Refereeden_US
dc.rights.holderAmerican Society for Microbiologyen_US
dc.contributor.departmentVirginia Tech. Department of Biological Sciencesen_US
dc.identifier.urlhttp://jcm.asm.org/content/51/6/1747en_US
dc.date.accessed2015-11-28en_US
dc.title.serialJournal of Clinical Microbiologyen_US
dc.identifier.doihttps://doi.org/10.1128/jcm.00186-13
dc.type.dcmitypeTexten_US


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