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dc.contributor.authorMoon, Sangjunen_US
dc.contributor.authorKim, Yun-Gonen_US
dc.contributor.authorDong, Lingshengen_US
dc.contributor.authorLombardi, Michaelen_US
dc.contributor.authorHaeggstrom, Edwarden_US
dc.contributor.authorJensen, Roderick V.en_US
dc.contributor.authorHsiao, Li-Lien_US
dc.contributor.authorDemirci, Utkanen_US
dc.date.accessioned2018-11-07T18:25:54Z
dc.date.available2018-11-07T18:25:54Z
dc.date.issued2011-03-11en_US
dc.identifier.othere17455en_US
dc.identifier.urihttp://hdl.handle.net/10919/85786
dc.description.abstractTechnologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and patterning of single cells from heterogeneous solutions as well as to keep them alive during the process due to a limited degree of control over single cell manipulation. Here, we present a microdroplet based method to isolate and pattern single cells from heterogeneous cell suspensions (10% target cell mixture), preserve viability of the extracted cells (97.0±0.8%), and obtain genomic information from isolated cells compared to the non-patterned controls. The cell encapsulation process is both experimentally and theoretically analyzed. Using the isolated cells, we identified 11 stem cell markers among 1000 genes and compare to the controls. This automated platform enabling high-throughput cell manipulation for subsequent genomic analysis employs fewer handling steps compared to existing methods.en_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherPLOSen_US
dc.rightsCreative Commons Attribution 4.0 Internationalen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0en_US
dc.titleDrop-on-Demand Single Cell Isolation and Total RNA Analysisen_US
dc.typeArticle - Refereeden_US
dc.description.versionPeer Revieweden_US
dc.title.serialPLOS ONEen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0017455en_US
dc.identifier.volume6en_US
dc.identifier.issue3en_US
dc.type.dcmitypeTexten_US
dc.identifier.pmid21412416en_US
dc.identifier.eissn1932-6203en_US


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International