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dc.contributor.authorWu, Zhanghanen
dc.contributor.authorWang, Hong-Weien
dc.contributor.authorMu, Weihuaen
dc.contributor.authorOuyang, Zhongcanen
dc.contributor.authorNogales, Evaen
dc.contributor.authorXing, Jianhuaen
dc.date.accessioned2018-11-14T14:44:43Zen
dc.date.available2018-11-14T14:44:43Zen
dc.date.issued2009-10-02en
dc.identifier.othere7291en
dc.identifier.urihttp://hdl.handle.net/10919/85843en
dc.description.abstractThe microtubule assembly process has been extensively studied, but the underlying molecular mechanism remains poorly understood. The structure of an artificially generated sheet polymer that alternates two types of lateral contacts and that directly converts into microtubules, has been proposed to correspond to the intermediate sheet structure observed during microtubule assembly. We have studied the self-assembly process of GMPCPP tubulins into sheet and microtubule structures using thermodynamic analysis and stochastic simulations. With the novel assumptions that tubulins can laterally interact in two different forms, and allosterically affect neighboring lateral interactions, we can explain existing experimental observations. At low temperature, the allosteric effect results in the observed sheet structure with alternating lateral interactions as the thermodynamically most stable form. At normal microtubule assembly temperature, our work indicates that a class of sheet structures resembling those observed at low temperature is transiently trapped as an intermediate during the assembly process. This work may shed light on the tubulin molecular interactions, and the role of sheet formation during microtubule assembly.en
dc.format.mimetypeapplication/pdfen
dc.language.isoen_USen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSimulations of Tubulin Sheet Polymers as Possible Structural Intermediates in Microtubule Assemblyen
dc.typeArticle - Refereeden
dc.description.versionPeer Revieweden
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.title.serialPLOS ONEen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0007291en
dc.identifier.volume4en
dc.identifier.issue10en
dc.type.dcmitypeTexten
dc.identifier.pmid19806219en
dc.identifier.eissn1932-6203en


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Creative Commons Attribution 4.0 International
License: Creative Commons Attribution 4.0 International