Now showing items 1-10 of 34
Genetic Reporter System for Positioning of Proteins at the Bacterial Pole
Spatial organization within bacteria is fundamental to many cellular processes, although the basic mechanisms underlying localization of proteins to specific sites within bacteria are poorly understood. The study of protein ...
Identification of Aspergillus fumigatus UDP-Galactopyranose Mutase Inhibitors
Aspergillus fumigatus is an opportunistic human pathogen responsible for deadly, invasive infections in immunocompromised patients. The A. fumigatus cell wall is a complex network of polysaccharides among them galactofuran, ...
Activation of PAD4 in NET formation.
Peptidylarginine deiminases, or PADs, convert arginine residues to the non-ribosomally encoded amino acid citrulline in a variety of protein substrates. PAD4 is expressed in granulocytes and is essential for the formation ...
Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor alpha target gene activation
Cofactors for estrogen receptor α (ERα) can modulate gene activity by posttranslationally modifying histone tails at target promoters. Here, we found that stimulation of ERα-positive cells with 17β-estradiol (E2) promotes ...
Fluorescence Polarization Binding Assay for Aspergillus fumigatus Virulence Factor UDP-Galactopyranose Mutase
Aspergillus fumigatus is an opportunistic human pathogenic fungus responsible for deadly lung infections in immunocompromised individuals. Galactofuranose (Galf) residues are essential components of the cell wall and play ...
Characterization of the Ornithine Hydroxylation Step in Albachelin Biosynthesis
<i>N</i>-Hydroxylating monooxygenases (NMOs) are involved in siderophore biosynthesis. Siderophores are high affinity iron chelators composed of catechol and hydroxamate functional groups that are synthesized and secreted ...
Learning Chemistry from Bacteria
Dr. Jon Clardy Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University All animals, including humans, originated and evolved on a planet already teeming with bacteria, and ...
Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond
A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of ...
Inhibition of PAD4 activity is sufficient to disrupt mouse and human NET formation.
PAD4 has been strongly implicated in the pathogenesis of autoimmune, cardiovascular and oncological diseases through clinical genetics and gene disruption in mice. New selective PAD4 inhibitors binding a calcium-deficient ...
Citrulination unravels stem cells
Maintenance of the pluripotent stem cell state is regulated by the post-translational modification of histones. The discovery that citrullination of the linker histone H1 is critical to this