Pine bark is the primary constituent of nursery container media (i.e., soilless substrate) in the eastern United States. Pine bark physical and hydraulic properties vary depending on the supplier due to source (e.g., lumber mill type) or methods of additional processing or aging. Pine bark can be processed via hammer milling or grinding before or after being aged from <= 1 month (fresh) to >= 6 month (aged). Additionally, bark is commonly amended with sand to alter physical properties and increase bulk density (D-b). Information is limited on physical or hydraulic differences of bark between varying sources or the effect of sand amendments. Pine bark physical and hydraulic properties from six commercial sources were compared as a function of age and amendment with sand. Aging bark, alone, had little effect on total porosity (TP), which remained at approximate to 80.5% (by volume). However, aging pine bark from <= 1 to >= 6 months shifted particle size from the coarse (>2 mm) to fine fraction (<0.5 mm), which increased container capacity (CC) 21.4% and decreased air space (AS) by 17.2% (by volume) regardless of source. The addition of sand to the substrate had a similar effect on particle size distribution to that of aging, increasing CC and D-b while decreasing AS. Total porosity decreased with the addition of sand. The magnitude of change in TP, AS, CC, and D-b from a nonamended pine bark substrate was greater with fine vs. coarse sand and varied by bark source. When comparing hydrological properties across three pine bark sources, readily available water content was unaffected; however, moisture characteristic curves (MCC) differed due to particle size distribution affecting the residual water content and subsequent shift from gravitational to either capillary or hygroscopic water. Similarly, hydraulic conductivity (i.e., ability to transfer water within the container) decreased with increasing particle size.