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dc.contributor.authorLuby, Elizabethen
dc.contributor.authorIbekwe, A. Marken
dc.contributor.authorZilles, Julieen
dc.contributor.authorPruden, Amyen
dc.description.abstractAgricultural ecosystems are of special interest for monitoring the potential for antibiotic resistance to spread through the environment and contribute to human exposure. Molecular methods, which target DNA, RNA, and other molecular components of bacterial cells, present certain advantages for characterizing and quantifying markers of antibiotic resistance and their horizontal gene transfer. These include rapid, unambiguous detection of targets; consistent results; and avoidance of culture bias. However, molecular methods are also subject to limitations that are not always clearly addressed or taken into consideration in the interpretation of scientific data. In particular, DNA-based methods do not directly assess viability or presence within an intact bacterial host, but such information may be inferred based on appropriate experimental design or in concert with complementary methods. The purpose of this review is to provide an overview of existing molecular methods for tracking antibiotic resistance in agricultural ecosystems, to define their strengths and weaknesses, and to recommend a path forward for future applications of molecular methods and standardized reporting in the literature. This will guide research along the farm-to-fork continuum and support comparability of the growing number of studies in the literature in a manner that informs management decisions and policy development.en
dc.description.sponsorshipAgriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2013-68003-21256]; USDA NIFAUnited States Department of Agriculture (USDA) [2014-05280]en
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.titleMolecular Methods for Assessment of Antibiotic Resistance in Agricultural Ecosystems: Prospects and Challengesen
dc.typeArticle - Refereeden
dc.contributor.departmentCivil and Environmental Engineeringen
dc.description.notesThis project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. Amy Pruden was also supported by USDA NIFA Grant no. 2014-05280.en
dc.title.serialJournal of Environmental Qualityen
dc.description.adminPublic domain – authored by a U.S. government employeeen

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Creative Commons CC0 1.0 Universal Public Domain Dedication
License: Creative Commons CC0 1.0 Universal Public Domain Dedication