FoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgf beta 1 signaling pathway

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dc.contributor.authorShi, Liminen
dc.contributor.authorTao, Zhipengen
dc.contributor.authorZheng, Louiseen
dc.contributor.authorYang, Jinyingen
dc.contributor.authorHu, Xinranen
dc.contributor.authorScott, Karenen
dc.contributor.authorde Kloet, Annetteen
dc.contributor.authorKrause, Ericen
dc.contributor.authorCollins, James F.en
dc.contributor.authorCheng, Zhiyongen
dc.date.accessioned2023-06-30T13:40:16Zen
dc.date.available2023-06-30T13:40:16Zen
dc.date.issued2023-07en
dc.description.abstractAdipose plasticity is critical for metabolic homeostasis. Adipocyte transdifferentiation plays an important role in adipose plasticity, but the molecular mechanism of transdifferentiation remains incompletely understood. Here we show that the transcription factor FoxO1 regulates adipose transdifferentiation by mediating Tgf beta 1 signaling pathway. Tgf beta 1 treatment induced whitening phenotype in beige adipocytes, reducing UCP1 and mitochondrial capacity and enlarging lipid droplets. Deletion of adipose FoxO1 (adO1KO) dampened Tgf beta 1 signaling by downregulating Tgfbr2 and Smad3 and induced browning of adipose tissue in mice, increasing UCP1 and mitochondrial content and activating metabolic pathways. Silencing FoxO1 also abolished the whitening effect of Tgf beta 1 on beige adipocytes. The adO1KO mice exhibited a significantly higher energy expenditure, lower fat mass, and smaller adipocytes than the control mice. The browning phenotype in adO1KO mice was associated with an increased iron content in adipose tissue, concurrent with upregulation of proteins that facilitate iron uptake (DMT1 and TfR1) and iron import into mitochondria (Mfrn1). Analysis of hepatic and serum iron along with hepatic iron-regulatory proteins (ferritin and ferroportin) in the adO1KO mice revealed an adipose tissue-liver crosstalk that meets the increased iron requirement for adipose browning. The FoxO1-Tgf beta 1 signaling cascade also underlay adipose browning induced by beta 3-AR agonist CL316243. Our study provides the first evidence of a FoxO1-Tgf beta 1 axis in the regulation of adipose browning-whitening trans-differentiation and iron influx, which sheds light on the compromised adipose plasticity in conditions of dysregulated FoxO1 and Tgf beta 1 signaling.en
dc.description.notesThis work was supported in part by the American Heart Association (18TPA34230082 to Z.C.) , and the USDA National Institute of Food and Agriculture (1020373 to Z.C.) .en
dc.description.sponsorshipAmerican Heart Association [18TPA34230082]; USDA National Institute of Food and Agriculture [1020373]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.redox.2023.102727en
dc.identifier.issn2213-2317en
dc.identifier.other102727en
dc.identifier.pmid37156218en
dc.identifier.urihttp://hdl.handle.net/10919/115606en
dc.identifier.volume63en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjecttranscription factor foxo1en
dc.subjectinsulin-resistanceen
dc.subjecttissueen
dc.subjectadipocytesen
dc.subjectmetabolismen
dc.subjectautophagyen
dc.subjectgrowthen
dc.subjectmiceen
dc.subjectlessonsen
dc.subjecthumansen
dc.titleFoxO1 regulates adipose transdifferentiation and iron influx by mediating Tgf beta 1 signaling pathwayen
dc.title.serialRedox Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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