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Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping

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dc.contributor.authorTabor, Kathryn M.en
dc.contributor.authorMarquart, Gregory D.en
dc.contributor.authorHurt, Christopheren
dc.contributor.authorSmith, Trevor S.en
dc.contributor.authorGeoca, Alexandra K.en
dc.contributor.authorBhandiwad, Ashwin A.en
dc.contributor.authorSubedi, Abhignyaen
dc.contributor.authorSinclair, Jennifer L.en
dc.contributor.authorRose, Hannah M.en
dc.contributor.authorPolys, Nicholas F.en
dc.contributor.authorBurgess, Harold A.en
dc.contributor.departmentComputer Scienceen
dc.date.accessioned2019-10-18T14:41:47Zen
dc.date.available2019-10-18T14:41:47Zen
dc.date.issued2019-02-08en
dc.description.abstractDecoding the functional connectivity of the nervous system is facilitated by transgenic methods that express a genetically encoded reporter or effector in specific neurons; however, most transgenic lines show broad spatiotemporal and cell-type expression. Increased specificity can be achieved using intersectional genetic methods which restrict reporter expression to cells that co-express multiple drivers, such as Gal4 and Cre. To facilitate intersectional targeting in zebrafish, we have generated more than 50 new Cre lines, and co-registered brain expression images with the Zebrafish Brain Browser, a cellular resolution atlas of 264 transgenic lines. Lines labeling neurons of interest can be identified using a web-browser to perform a 3D spatial search (zbbrowser.com). This resource facilitates the design of intersectional genetic experiments and will advance a wide range of precision circuit-mapping studies.en
dc.description.notesEunice Kennedy Shriver National Institute of Child Health and Human Development 1ZIAHD008884-04 Harold A Burgess; Virginia Tech Advanced Research Computing Nicholas F Polysen
dc.description.sponsorshipEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) [1ZIAHD008884-04]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.7554/eLife.42687en
dc.identifier.issn2050-084Xen
dc.identifier.othere42687en
dc.identifier.pmid30735129en
dc.identifier.urihttp://hdl.handle.net/10919/94637en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectin-vivoen
dc.subjectgene-expressionen
dc.subjectoptical controlen
dc.subjectknock-inen
dc.subjectgal4en
dc.subjectdissectionen
dc.subjectsystemen
dc.subjectarchitectureen
dc.subjectpatternsen
dc.subjecttoolsen
dc.titleBrain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mappingen
dc.title.serialeLifeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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