Phytophthora sojae Avirulence Effector Avr3b is a Secreted NADH and ADP-ribose Pyrophosphorylase that Modulates Plant Immunity

Simple item page
dc.contributorVirginia Techen
dc.contributor.authorDong, Suomengen
dc.contributor.authorYin, Weixiaoen
dc.contributor.authorKong, Guanghuien
dc.contributor.authorYang, Xinyuen
dc.contributor.authorQutob, Dinahen
dc.contributor.authorChen, Qingheen
dc.contributor.authorKale, Shiv D.en
dc.contributor.authorSui, Yangyangen
dc.contributor.authorZhang, Zhengguangen
dc.contributor.authorDou, Daolongen
dc.contributor.authorZheng, Xiaoboen
dc.contributor.authorGijzen, Marken
dc.contributor.authorTyler, Brett M.en
dc.contributor.authorWang, Yuanchaoen
dc.date.accessed2014-04-03en
dc.date.accessioned2014-04-09T15:07:21Zen
dc.date.available2014-04-09T15:07:21Zen
dc.date.issued2011-11-10en
dc.description.abstractPlants have evolved pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) to protect themselves from infection by diverse pathogens. Avirulence (Avr) effectors that trigger plant ETI as a result of recognition by plant resistance (R) gene products have been identified in many plant pathogenic oomycetes and fungi. However, the virulence functions of oomycete and fungal Avr effectors remain largely unknown. Here, we combined bioinformatics and genetics to identify Avr3b, a new Avr gene from Phytophthora sojae, an oomycete pathogen that causes soybean root rot. Avr3b encodes a secreted protein with the RXLR host-targeting motif and C-terminal W and Nudix hydrolase motifs. Some isolates of P. sojae evade perception by the soybean R gene Rps3b through sequence mutation in Avr3b and lowered transcript accumulation. Transient expression of Avr3b in Nicotiana benthamiana increased susceptibility to P. capsici and P. parasitica, with significantly reduced accumulation of reactive oxygen species (ROS) around invasion sites. Biochemical assays confirmed that Avr3b is an ADP-ribose/NADH pyrophosphorylase, as predicted from the Nudix motif. Deletion of the Nudix motif of Avr3b abolished enzyme activity. Mutation of key residues in Nudix motif significantly impaired Avr3b virulence function but not the avirulence activity. Some Nudix hydrolases act as negative regulators of plant immunity, and thus Avr3b might be delivered into host cells as a Nudix hydrolase to impair host immunity. Avr3b homologues are present in several sequenced Phytophthora genomes, suggesting that Phytophthora pathogens might share similar strategies to suppress plant immunity.en
dc.description.sponsorshipThis work was supported by the National Basic Research Program of China (973 Program; number 2009CB119200) to Y. W and X. Z., National Natural Science Foundation of China (30900932) to S.D. and (30671345) to Y. W., the Special Fund for Agro-scientific Research in the Public Interest (3–20) from China to Y.W., and Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and 111 International Cooperation grant (B07030) to Nanjing Agricultural University from the Chinese government, and in part by US National Science Foundation grant IOS-0924861 to B.M.T. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.citationDong S, Yin W, Kong G, Yang X, Qutob D, et al. (2011) Phytophthora sojae Avirulence Effector Avr3b is a Secreted NADH and ADP-ribose Pyrophosphorylase that Modulates Plant Immunity. PLoS Pathog 7(11): e1002353. doi:10.1371/journal.ppat.1002353en
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1002353en
dc.identifier.issn1553-7374en
dc.identifier.urihttp://hdl.handle.net/10919/47007en
dc.identifier.urlhttp://su8bj7jh4j.search.serialssolutions.com/?V=1.0&S=I_M&C=1553-7374en
dc.language.isoen_USen
dc.publisherPLOS Pathogensen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCell deathen
dc.subjectDNA sequenceen
dc.subjectGenetic ocien
dc.subjectPhytophthoraen
dc.subjectPlant pthogensen
dc.subjectSeedlingsen
dc.subjectSequence motif analysisen
dc.subjectSoybeanen
dc.titlePhytophthora sojae Avirulence Effector Avr3b is a Secreted NADH and ADP-ribose Pyrophosphorylase that Modulates Plant Immunityen
dc.title.serialPLoS Pathogensen
dc.typeArticle - Refereeden

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.ppat.1002353.pdf
Size:
2.54 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, Fralin Life Sciences Institute