Genotypic Variation in Resistance Gene-Mediated Calcium Signaling and Hormonal Signaling Involved in Effector-Triggered Immunity or Disease Susceptibility in the Xanthomonas campestris pv. Campestris-Brassica napus Pathosystem

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dc.contributor.authorAl Mamun, Mden
dc.contributor.authorIslam, Md Tabibulen
dc.contributor.authorLee, Bok-Ryeen
dc.contributor.authorLa, Van Hienen
dc.contributor.authorBae, Dong-Wonen
dc.contributor.authorKim, Tae-Hwanen
dc.date.accessioned2020-05-13T13:29:09Zen
dc.date.available2020-05-13T13:29:09Zen
dc.date.issued2020-03en
dc.description.abstractTo characterize cultivar variation in resistance gene (R-gene)-mediated calcium signaling and hormonal regulation in effector-triggered immunity (ETI) and disease susceptibility, Xanthomonas campestris pv. campestris (Xcc) was inoculated in two Brassica napus cultivars (cvs. Capitol and Mosa). At 14 days post inoculation (DPI) with Xcc, there was a necrotic lesion in cv. Mosa along with the significant accumulation of H2O2 and malondialdehyde (MDA), whereas no visual symptom was observed in cv. Capitol. The cultivar variations in the R-gene expressions were found in response to Xcc. ZAR1 is a coiled-coil-nucleotide binding site-leucine-rich repeat (CC-NB-LRR)-type R-gene that is significantly induced in cv. Capitol, whereas toll/interleukin-1 receptor-nucleotide binding site-leucine-rich repeat (TIR-NB-LRR)-type R-gene, TAO1, is significantly upregulated in cv. Mosa Xcc-inoculated plants. The defense-related gene's non-race-specific disease resistance 1 (NDR1) and mitogen-activated protein kinase 6 (MAPK6) were enhanced, whereas calcium-dependent protein kinase (CDPK5) and calcium-sensing protein 60g (CBP60g) were depressed in cv. Capitol Xcc inoculated plants, and opposite results were found in cv. Mosa. The calcium-sensing receptor (CAS), calmodulin (CaM), expression was induced in both the cultivars. However, the CAS induction rate was much higher in cv. Mosa than in cv. Capitol in response to Xcc. The phytohormone salicylic acid (SA) and jasmonic acid (JA) levels were significantly higher in cv. Capitol along with the enhanced SA receptors (NPR3 and NPR4) and JA synthesis and signaling-related gene expression (LOX2, PDF1.2), whereas the JA level was significantly lower in cv. Mosa Xcc inoculated plants. The SA synthesis and signaling-related genes (ICS1, NPR1) and SA were present at higher levels in cv. Mosa; additionally, the SA level present was much higher in the susceptible cultivar (cv. Mosa) than in the resistant cultivar (cv. Capitol) in response to Xcc. These results indicate that ZAR1 mediated the coordinated action of SA and JA synthesis and signaling to confirm ETI, whereas TAO1 enhanced the synthesis of SA through CAS and CBP60g to antagonize JA synthesis and signaling to cause disease susceptibility in the Brassica napus-Xcc pathosystem.en
dc.description.notesThis research was funded by the National Research Foundation (NRF-2019R1A2C1089340).en
dc.description.sponsorshipNational Research Foundation [NRF-2019R1A2C1089340]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/plants9030303en
dc.identifier.eissn2223-7747en
dc.identifier.issue3en
dc.identifier.other303en
dc.identifier.pmid32121557en
dc.identifier.urihttp://hdl.handle.net/10919/98236en
dc.identifier.volume9en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectjasmonic aciden
dc.subjectsalicylic aciden
dc.subjectZAR1en
dc.subjectTAO1en
dc.subjectXanthomonas campestris pven
dc.subjectcampestrisen
dc.titleGenotypic Variation in Resistance Gene-Mediated Calcium Signaling and Hormonal Signaling Involved in Effector-Triggered Immunity or Disease Susceptibility in the Xanthomonas campestris pv. Campestris-Brassica napus Pathosystemen
dc.title.serialPlantsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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