The application of microsatellite DNA markers for forensic analysis of koi carp (Cyprinus carpio)

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dc.contributor.authorGrobler, J. P.en
dc.contributor.authorKotze, A.en
dc.contributor.authorSwart, H.en
dc.contributor.authorHallerman, Eric M.en
dc.contributor.departmentFish and Wildlife Conservationen
dc.date.accessed2015-03-31en
dc.date.accessioned2015-03-31T17:00:38Zen
dc.date.available2015-03-31T17:00:38Zen
dc.date.issued2005en
dc.description.abstractWe describe the use of microsatellite DNA markers, previously developed for common carp, to investigate a forensic case involving ornamental koi carp. Two South African breeders offered koi strains for sale, which a third breeder claimed were taken illegally from his ponds. Screening of four microsatellite markers provided polymorphic results for koi, demonstrating their applicability for forensic studies. Amplification product sizes were comparable to those published for common carp at three of the four loci. We observed higher allelic diversity among koi carp than had been reported for a panel of inbred common carp. Coefficients of population differentiation showed no significant differences between the populations involved. Consequently, our analyses could not convincingly prove or disprove foul play. The latter ambiguity was most likely the result of sampling constraints rather than the suitability of the markers. Our results provide a foundation for future application of microsatellite markers for forensic investigation, marker-assisted breeding, and population diversity analysis in koi.en
dc.identifier.citationGrobler, J. P., Kotze, A., Swart, H., & Hallerman, E. M. (2005). The application of microsatellite DNA markers for forensic analysis of koi carp (Cyprinus carpio). South African Journal of Science, 101(1-2), 19-21.en
dc.identifier.issn0038-2353en
dc.identifier.urihttp://hdl.handle.net/10919/51658en
dc.language.isoen_USen
dc.publisherAcademy of Science of South Africaen
dc.rightsCreative Commons Attribution 2.5 South Africaen
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/za/en
dc.subjectMicrosatellite DNA markersen
dc.subjectKoi carpen
dc.subjectFisheries scienceen
dc.subjectFisheries geneticsen
dc.titleThe application of microsatellite DNA markers for forensic analysis of koi carp (Cyprinus carpio)en
dc.title.serialSouth African Journal of Scienceen
dc.typeArticle - Refereeden

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