Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform

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dc.contributor.authorSkardal, Aleksanderen
dc.contributor.authorMurphy, Sean V.en
dc.contributor.authorDevarasetty, Maheshen
dc.contributor.authorMead, Ivyen
dc.contributor.authorKang, Hyun-Wooken
dc.contributor.authorSeol, Young-Joonen
dc.contributor.authorZhang, Yu Shrikeen
dc.contributor.authorShin, Su-Ryonen
dc.contributor.authorZhao, Liangen
dc.contributor.authorAleman, Julioen
dc.contributor.authorHall, Adam R.en
dc.contributor.authorShupe, Thomas D.en
dc.contributor.authorKleensang, Andreen
dc.contributor.authorDokmeci, Mehmet R.en
dc.contributor.authorLee, Sang Jinen
dc.contributor.authorJackson, John D.en
dc.contributor.authorYoo, James J.en
dc.contributor.authorHartung, Thomasen
dc.contributor.authorKhademhosseini, Alien
dc.contributor.authorSoker, Shayen
dc.contributor.authorBishop, Colin E.en
dc.contributor.authorAtala, Anthonyen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2019-01-04T15:46:39Zen
dc.date.available2019-01-04T15:46:39Zen
dc.date.issued2017-08-18en
dc.description.abstractMany drugs have progressed through preclinical and clinical trials and have been available - for years in some cases -before being recalled by the FDA for unanticipated toxicity in humans. One reason for such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs and their response to drug compounds. Moreover, tissues in the body do not exist in isolation, but reside in a highly integrated and dynamically interactive environment, in which actions in one tissue can affect other downstream tissues. Few engineered model systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far reflected the interactive nature of the human body. To address this challenge, we have developed an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a closed circulatory perfusion system, facilitating inter-organ responses. We describe a three-tissue organ-on-a-chip system, comprised of liver, heart, and lung, and highlight examples of inter-organ responses to drug administration. We observe drug responses that depend on inter-tissue interaction, illustrating the value of multiple tissue integration for in vitro study of both the efficacy of and side effects associated with candidate drugs.en
dc.description.notesThe authors gratefully thank Dr. Pedro Baptista for aid in the drug metabolism studies, Steven Forsythe and Meiei Wan for technical aid in maintaining liver and cardiac organoid viability, and Dipasri Konar and Katherine Crowell for technical aid in the lung-on-a-chip operation. The authors gratefully acknowledge funding by the Defense Threat Reduction Agency (DTRA) under Space and Naval Warfare Systems Center Pacific (SSC PACIFIC) Contract No. N6601-13-C-2027. The publication of this material does not constitute approval by the government of the findings or conclusions herein. Proteomics and Metabolomics Core Lab services are supported by the Comprehensive Cancer Center of Wake Forest University NCI CCSG P30CA012197 grant.en
dc.description.sponsorshipDefense Threat Reduction Agency (DTRA) under Space and Naval Warfare Systems Center Pacific (SSC PACIFIC) [N6601-13-C-2027]; Comprehensive Cancer Center of Wake Forest University NCI CCSG [P30CA012197]en
dc.format.extent16en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-017-08879-xen
dc.identifier.issn2045-2322en
dc.identifier.other8837en
dc.identifier.pmid28821762en
dc.identifier.urihttp://hdl.handle.net/10919/86606en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectdrug screening applicationsen
dc.subjectdisease-modelsen
dc.subjecttumoren
dc.subjectliveren
dc.subjectbleomycinen
dc.subjectcardiotoxicityen
dc.subjectchemotherapyen
dc.subjectdiscoveryen
dc.subjectspheroidsen
dc.subjecthydrogelsen
dc.titleMulti-tissue interactions in an integrated three-tissue organ-on-a-chip platformen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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