Rule-Based Design of Plant Expression Vectors Using GenoCAD

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dc.contributor.authorColl, Annaen
dc.contributor.authorWilson, Mandy L.en
dc.contributor.authorGruden, Kristinaen
dc.contributor.authorPeccoud, Jeanen
dc.date.accessioned2018-09-24T14:37:09Zen
dc.date.available2018-09-24T14:37:09Zen
dc.date.issued2015-07-06en
dc.description.abstractPlant synthetic biology requires software tools to assist on the design of complex multi-genic expression plasmids. Here a vector design strategy to express genes in plants is formalized and implemented as a grammar in GenoCAD, a Computer-Aided Design software for synthetic biology. It includes a library of plant biological parts organized in structural categories and a set of rules describing how to assemble these parts into large constructs. Rules developed here are organized and divided into three main subsections according to the aim of the final construct: protein localization studies, promoter analysis and protein-protein interaction experiments. The GenoCAD plant grammar guides the user through the design while allowing users to customize vectors according to their needs. Therefore the plant grammar implemented in GenoCAD will help plant biologists take advantage of methods from synthetic biology to design expression vectors supporting their research projects.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0132502en
dc.identifier.eissn1932-6203en
dc.identifier.issue7en
dc.identifier.othere0132502en
dc.identifier.pmid26148190en
dc.identifier.urihttp://hdl.handle.net/10919/85120en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleRule-Based Design of Plant Expression Vectors Using GenoCADen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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