Functional and Predictive Structural Characterization of WRINKLED2, A Unique Oil Biosynthesis Regulator in Avocado

Simple item page
dc.contributor.authorBehera, Jyoti R.en
dc.contributor.authorRahman, Md. Mahbuburen
dc.contributor.authorBhatia, Shinaen
dc.contributor.authorShockey, Jayen
dc.contributor.authorKilaru, Arunaen
dc.date.accessioned2021-11-18T17:41:58Zen
dc.date.available2021-11-18T17:41:58Zen
dc.date.issued2021-06-08en
dc.description.abstractWRINKLED1 (WRI1), a member of the APETALA2 (AP2) class of transcription factors regulates fatty acid biosynthesis and triacylglycerol (TAG) accumulation in plants. Among the four known Arabidopsis WRI1 paralogs, only WRI2 was unable to complement and restore fatty acid content in wri1-1 mutant seeds. Avocado (Persea americana) mesocarp, which accumulates 60-70% dry weight oil content, showed high expression levels for orthologs of WRI2, along with WRI1 and WRI3, during fruit development. While the role of WRI1 as a master regulator of oil biosynthesis is well-established, the function of WRI1 paralogs is poorly understood. Comprehensive and comparative in silico analyses of WRI1 paralogs from avocado (a basal angiosperm) with higher angiosperms Arabidopsis (dicot), maize (monocot) revealed distinct features. Predictive structural analyses of the WRI orthologs from these three species revealed the presence of AP2 domains and other highly conserved features, such as intrinsically disordered regions associated with predicted PEST motifs and phosphorylation sites. Additionally, avocado WRI proteins also contained distinct features that were absent in the nonfunctional Arabidopsis ortholog AtWRI2. Through transient expression assays, we demonstrated that both avocado WRI1 and WRI2 are functional and drive TAG accumulation in Nicotiana benthamiana leaves. We predict that the unique features and activities of ancestral PaWRI2 were likely lost in orthologous genes such as AtWRI2 during evolution and speciation, leading to at least partial loss of function in some higher eudicots. This study provides us with new targets to enhance oil biosynthesis in plants.en
dc.description.notesThis research was supported in part by minor and major grants from research and development committee, East Tennessee State University (ETSU) awarded to AK. Additionally, JB, MR, and SB received funds from School of Graduate Studies, ETSU. SB and MR received Sigma Xi GIAR Award. SB also received Fraley's Memorial Research Award from the Department of Biological Sciences, ETSU.en
dc.description.sponsorshipresearch and development committee, East Tennessee State University (ETSU); School of Graduate Studies, ETSU; Sigma Xi GIAR Award; Department of Biological Sciences, ETSUen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2021.648494en
dc.identifier.issn1664-462Xen
dc.identifier.other648494en
dc.identifier.pmid34168663en
dc.identifier.urihttp://hdl.handle.net/10919/106678en
dc.identifier.volume12en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAP2 domainen
dc.subjectArabidopsis thalianaen
dc.subjectfatty acidsen
dc.subjectmesocarpen
dc.subjectPersea americanaen
dc.subjecttranscription factoren
dc.subjecttriacylglycerolen
dc.subjectWRINKLED1en
dc.titleFunctional and Predictive Structural Characterization of WRINKLED2, A Unique Oil Biosynthesis Regulator in Avocadoen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fpls-12-648494.pdf
Size:
2.3 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Scholarly Works, Biological Systems Engineering