Treatment of hemophilia with human Factor IX produced in mammary tissue of transgenic mammals

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dc.contributor.assigneeThe American Red Crossen
dc.contributor.assigneeVirginia Tech Intellectual Properties, Inc.en
dc.contributor.inventorDrohan, William N.en
dc.contributor.inventorVelander, William H.en
dc.contributor.inventorLubon, Henryken
dc.contributor.inventorJohnson, Mary Ann H.en
dc.contributor.inventorJohnson, John L.en
dc.date.accessed2016-08-19en
dc.date.accessioned2016-08-24T17:54:47Zen
dc.date.available2016-08-24T17:54:47Zen
dc.date.filed2005-04-29en
dc.date.issued2008-09-02en
dc.description.abstractRecombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5′-untranslated and 3′-untranslated region, which is substituted with a 5-′ and 3′-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described.en
dc.format.mimetypeapplication/pdfen
dc.identifier.applicationnumber11117705en
dc.identifier.patentnumber7419948en
dc.identifier.urihttp://hdl.handle.net/10919/72590en
dc.identifier.urlhttp://pimg-fpiw.uspto.gov/fdd/48/199/074/0.pdfen
dc.language.isoen_USen
dc.publisherUnited States Patent and Trademark Officeen
dc.subject.cpcA01K67/0275en
dc.subject.cpcA01K67/0278en
dc.subject.cpcA01K2207/15en
dc.subject.cpcA01K2217/00en
dc.subject.cpcA01K2217/05en
dc.subject.cpcA01K2227/105en
dc.subject.cpcA01K2227/108en
dc.subject.cpcA01K2267/01en
dc.subject.cpcA01K2267/03en
dc.subject.cpcA61K38/36en
dc.subject.cpcC12N15/8509en
dc.subject.cpcC12N9/644en
dc.subject.cpcC12Y304/21022en
dc.subject.uspc512/12en
dc.subject.uspcother530/381en
dc.subject.uspcother800/7en
dc.titleTreatment of hemophilia with human Factor IX produced in mammary tissue of transgenic mammalsen
dc.typePatenten
dc.type.dcmitypeTexten
dc.type.patenttypeutilityen
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