Activity of bacteria isolated from bats against Pseudogymnoascus destructans in China

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dc.contributor.authorLi, Zhongleen
dc.contributor.authorLi, Aoqiangen
dc.contributor.authorHoyt, Joseph R.en
dc.contributor.authorDai, Wentaoen
dc.contributor.authorLeng, Haixiaen
dc.contributor.authorLi, Yanfeien
dc.contributor.authorLi, Weien
dc.contributor.authorLiu, Senen
dc.contributor.authorJin, Longruen
dc.contributor.authorSun, Kepingen
dc.contributor.authorFeng, Jiangen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2021-05-20T12:08:16Zen
dc.date.available2021-05-20T12:08:16Zen
dc.date.issued2021-02en
dc.description.abstractWhite-nose syndrome, a disease that is caused by the psychrophilic fungus Pseudogymnoascus destructans, has threatened several North America bat species with extinction. Recent studies have shown that East Asian bats are infected with P. destructans but show greatly reduced infections. While several factors have been found to contribute to these reduced infections, the role of specific microbes in limiting P. destructans growth remains unexplored. We isolated three bacterial strains with the ability to inhibit P. destructans, namely, Pseudomonas yamanorum GZD14026, Pseudomonas brenneri XRD11711 and Pseudomonas fragi GZD14479, from bats in China. Pseudomonas yamanorum, with the highest inhibition score, was selected to extract antifungal active substance. Combining mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy analyses, we identified the active compound inhibiting P. destructans as phenazine-1-carboxylic acid (PCA), and the minimal inhibitory concentration (MIC) was 50.12 mu g ml(-1). Whole genome sequencing also revealed the existence of PCA biosynthesis gene clusters. Gas chromatography-mass spectrometry (GC-MS) analysis identified volatile organic compounds. The results indicated that 10 ppm octanoic acid, 100 ppm 3-tert-butyl-4-hydroxyanisole (isoprenol) and 100 ppm 3-methyl-3-buten-1-ol (BHA) inhibited the growth of P. destructans. These results support that bacteria may play a role in limiting the growth of P. destructans on bats.en
dc.description.notesThis work was supported by the National Natural Science Foundation of China (grant numbers 31961123001, 31770403, 31570390 and 31670390), Jilin Provincial Natural Science Foundation (grant number 20180101272JC), and the Program for Introducing Talents to Universities (B16011) and the US National Science Foundation (DEB-1911853).en
dc.description.sponsorshipNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31961123001, 31770403, 31570390, 31670390]; Jilin Provincial Natural Science Foundation [20180101272JC]; Program for Introducing Talents to Universities [B16011]; US National Science FoundationNational Science Foundation (NSF) [DEB-1911853]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/1751-7915.13765en
dc.identifier.issn1751-7915en
dc.identifier.pmid33559264en
dc.identifier.urihttp://hdl.handle.net/10919/103394en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.titleActivity of bacteria isolated from bats against Pseudogymnoascus destructans in Chinaen
dc.title.serialMicrobial Biotechnologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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