Adsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Sera

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dc.contributor.authorSolivio, Morwena J.en
dc.contributor.authorLess, Rebekahen
dc.contributor.authorRynes, Mathew L.en
dc.contributor.authorKramer, Marcusen
dc.contributor.authorAksan, Alptekinen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2019-01-23T14:27:29Zen
dc.date.available2019-01-23T14:27:29Zen
dc.date.issued2016-04-12en
dc.description.abstractDespite abundant research conducted on cancer biomarker discovery and validation, to date, less than two-dozen biomarkers have been approved by the FDA for clinical use. One main reason is attributed to inadvertent use of low quality biospecimens in biomarker research. Most proteinaceous biomarkers are extremely susceptible to pre-analytical factors such as collection, processing, and storage. For example, cryogenic storage imposes very harsh chemical, physical, and mechanical stresses on biospecimens, significantly compromising sample quality. In this communication, we report the development of an electrospun lyoprotectant matrix and isothermal vitrification methodology for non-cryogenic stabilization and storage of liquid biospecimens. The lyoprotectant matrix was mainly composed of trehalose and dextran (and various low concentration excipients targeting different mechanisms of damage), and it was engineered to minimize heterogeneity during vitrification. The technology was validated using five biomarkers; LDH, CRP, PSA, MMP-7, and C3a. Complete recovery of LDH, CRP, and PSA levels was achieved post-rehydration while more than 90% recovery was accomplished for MMP-7 and C3a, showing promise for isothermal vitrification as a safe, efficient, and low-cost alternative to cryogenic storage.en
dc.description.notesThe authors thank Ms. Goeun Heo for her valuable assistance with electrospinning. This research was supported by an NIH-NCI grant (5R21CA157298) to A.A.en
dc.description.sponsorshipNIH-NCI grant [5R21CA157298]en
dc.format.extent14en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep24186en
dc.identifier.issn2045-2322en
dc.identifier.other24186en
dc.identifier.pmid27068126en
dc.identifier.urihttp://hdl.handle.net/10919/86850en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectc-reactive proteinen
dc.subjectlactate-dehydrogenaseen
dc.subjectinduced denaturationen
dc.subjectbiomarker discoveryen
dc.subjectcold denaturationen
dc.subjectfrozen stateen
dc.subjectstabilizationen
dc.subjectcanceren
dc.subjectblooden
dc.subjectvitrificationen
dc.titleAdsorbing/dissolving Lyoprotectant Matrix Technology for Non-cryogenic Storage of Archival Human Seraen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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