Production of lysosomal enzymes in plant-based expression systems

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dc.contributor.assigneeCroptech Development Corporationen
dc.contributor.assigneeVirginia Tech Intellectual Properties, Inc.en
dc.contributor.inventorCramer, Carole Lynen
dc.contributor.inventorWeissenborn, Deborah Louiseen
dc.contributor.inventorRadin, David N.en
dc.contributor.inventorOishi, Karen K.en
dc.date.accessed2016-08-19en
dc.date.accessioned2016-08-24T17:54:06Zen
dc.date.available2016-08-24T17:54:06Zen
dc.date.filed1996-09-13en
dc.date.issued1999-07-27en
dc.description.abstractThe invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human hGC and IDUA nucleotide sequences produced enzymatically active modified human glucocerebrosidase and human .alpha.-L-iduronidase. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.en
dc.format.mimetypeapplication/pdfen
dc.identifier.applicationnumber8713928en
dc.identifier.patentnumber5929304en
dc.identifier.urihttp://hdl.handle.net/10919/72395en
dc.identifier.urlhttp://pimg-fpiw.uspto.gov/fdd/04/293/059/0.pdfen
dc.language.isoen_USen
dc.publisherUnited States Patent and Trademark Officeen
dc.subject.cpcA61K38/00en
dc.subject.cpcC12Y302/01045en
dc.subject.cpcC12N9/2402en
dc.subject.cpcC12N15/8237en
dc.subject.cpcC12N15/8257en
dc.subject.cpcC12Y302/01018en
dc.subject.cpcC12Y302/01022en
dc.subject.cpcC12Y302/01024en
dc.subject.cpcC12Y302/01049en
dc.subject.cpcC12Y302/01076en
dc.subject.uspc435/69.1en
dc.subject.uspcother435/183en
dc.subject.uspcother435/206en
dc.subject.uspcother435/320.1en
dc.subject.uspcother435/410en
dc.subject.uspcother435/414en
dc.subject.uspcother536/23.1en
dc.subject.uspcother536/23.2en
dc.subject.uspcother536/24.1en
dc.subject.uspcother800/288en
dc.subject.uspcother800/278en
dc.subject.uspcother800/287en
dc.subject.uspcother800/294en
dc.subject.uspcother800/295en
dc.subject.uspcother800/317.3en
dc.titleProduction of lysosomal enzymes in plant-based expression systemsen
dc.typePatenten
dc.type.dcmitypeTexten
dc.type.patenttypeutilityen

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