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Response of the Hepatic Transcriptome to Aflatoxin B-1 in Domestic Turkey (Meleagris gallopavo)

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dc.contributor.authorMonson, Melissa S.en
dc.contributor.authorSettlage, Robert E.en
dc.contributor.authorMcMahon, Kevin W.en
dc.contributor.authorMendoza, Kristelle M.en
dc.contributor.authorRawal, Sumiten
dc.contributor.authorEl-Nezami, Hani S.en
dc.contributor.authorCoulombe, Roger A.en
dc.contributor.authorReed, Kent M.en
dc.date.accessioned2018-10-04T14:11:22Zen
dc.date.available2018-10-04T14:11:22Zen
dc.date.issued2014-06-30en
dc.description.abstractDietary exposure to aflatoxin B1 (AFB1) is detrimental to avian health and leads to major economic losses for the poultry industry. AFB1 is especially hepatotoxic in domestic turkeys (Meleagris gallopavo), since these birds are unable to detoxify AFB1 by glutathione-conjugation. The impacts of AFB1 on the turkey hepatic transcriptome and the potential protection from pretreatment with a Lactobacillus-based probiotic mixture were investigated through RNA-sequencing. Animals were divided into four treatment groups and RNA was subsequently recovered from liver samples. Four pooled RNA-seq libraries were sequenced to produce over 322 M reads totaling 13.8 Gb of sequence. Approximately 170,000 predicted transcripts were de novo assembled, of which 803 had significant differential expression in at least one pair-wise comparison between treatment groups. Functional analysis linked many of the transcripts significantly affected by AFB1 exposure to cancer, apoptosis, the cell cycle or lipid regulation. Most notable were transcripts from the genes encoding E3 ubiquitin-protein ligase Mdm2, osteopontin, S-adenosylmethionine synthase isoform type-2, and lipoprotein lipase. Expression was modulated by the probiotics, but treatment did not completely mitigate the effects of AFB1. Genes identified through transcriptome analysis provide candidates for further study of AFB1 toxicity and targets for efforts to improve the health of domestic turkeys exposed to AFB1.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0100930en
dc.identifier.eissn1932-6203en
dc.identifier.issue6en
dc.identifier.othere100930en
dc.identifier.pmid24979717en
dc.identifier.urihttp://hdl.handle.net/10919/85227en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleResponse of the Hepatic Transcriptome to Aflatoxin B-1 in Domestic Turkey (Meleagris gallopavo)en
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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