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Detection of Salmonella spp. Using a Generic and Differential FRET-PCR

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dc.contributor.authorZhang, Jileien
dc.contributor.authorWei, Lanjingen
dc.contributor.authorKelly, Patricken
dc.contributor.authorFreeman, Marken
dc.contributor.authorJaegerson, Kirstenen
dc.contributor.authorGong, Jiansenen
dc.contributor.authorXu, Buen
dc.contributor.authorPan, Zhimingen
dc.contributor.authorXu, Chuanlingen
dc.contributor.authorWang, Chengmingen
dc.date.accessioned2018-10-17T14:56:40Zen
dc.date.available2018-10-17T14:56:40Zen
dc.date.issued2013-10-16en
dc.description.abstractTo facilitate the detection of Salmonella and to be able to rapidly and conveniently determine the species/subspecies present, we developed and tested a generic and differential FRET-PCR targeting their tetrathionate reductase response regulator gene. The differential pan-Salmonella FRET-PCR we developed successfully detected seven plasmids that contained partial sequences of S. bongori and the six S. enterica subspecies. The detection limit varied from ∼5 copies of target gene/per PCR reaction for S. enterica enterica to ∼200 for S. bongori. Melting curve analysis demonstrated a Tm of ∼68°C for S. enterica enterica, ∼62.5°C for S. enterica houtenae and S. enterica diarizonae, ∼57°C for S. enterica indica, and ∼54°C for S. bongori, S. enterica salamae and S. enterica arizonae. The differential pan-Salmonella FRET-PCR also detected and determined the subspecies of 4 reference strains and 47 Salmonella isolated from clinically ill birds or pigs. Finally, we found it could directly detect and differentiate Salmonella in feline (5/50 positive; 10%; one S. enterica salamae and 4 S. enterica enterica) and canine feces (15/114 positive; 13.2%; all S. enterica enterica). The differential pan-Salmonella FRET-PCR failed to react with 96 non-Salmonella bacterial strains. Our experiments show the differential pan-Salmonella FRET-PCR we developed is a rapid, sensitive and specific method to detect and differentiate Salmonella.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0076053en
dc.identifier.eissn1932-6203en
dc.identifier.issue10en
dc.identifier.othere76053en
dc.identifier.pmid24146814en
dc.identifier.urihttp://hdl.handle.net/10919/85392en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleDetection of Salmonella spp. Using a Generic and Differential FRET-PCRen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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