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The Hsp70-like StkA functions between T4P and Dif signaling proteins as a negative regulator of exopolysaccharide in Myxococcus xanthus

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dc.contributorVirginia Tech. Department of Biological Sciencesen
dc.contributor.authorMoak, Pamela L.en
dc.contributor.authorBlack, Wesley P.en
dc.contributor.authorWallace, Regina A.en
dc.contributor.authorLi, Zhuoen
dc.contributor.authorYang, Zhaominen
dc.contributor.departmentBiological Sciencesen
dc.contributor.editorEtchevers, Heatheren
dc.date.accessed2016-02-12en
dc.date.accessioned2016-02-16T08:03:27Zen
dc.date.available2016-02-16T08:03:27Zen
dc.date.issued2015-02-03en
dc.description.abstractMyxococcus xanthus displays a form of surface motility known as social (S) gliding. It is mediated by the type IV pilus (T4P) and requires the exopolysaccharide (EPS) to function. It is clear that T4P retraction powers S motility. EPS on a neighboring cell or deposited on a gliding surface is proposed to anchor the distal end of a pilus and trigger T4P retraction at its proximal end. Inversely, T4P has been shown to regulate EPS production upstream of the Dif signaling pathway. Here we describe the isolation of two Tn insertions at the stk locus which had been known to play roles in cellular cohesion and formation of cell groups. An insertion in stkA (MXAN_3474) was identified based on its ability to restore EPS to a pilA deletion mutant. The stkA encodes a DnaK or Hsp70 homolog and it is upstream of stkB (MXAN_3475) and stkC (MXAN_3476). A stkB insertion was identified in a separate genetic screen because it eliminated EPS production of an EPS+ parental strain. Our results with in-frame deletions of these three stk genes indicated that the stkA mutant produced increased level of EPS while stkB and stkC mutants produced less EPS relative to the wild type. S motility and developmental aggregation were affected by deletions of stkA and stkB but only minimally by the deletion of stkC. Genetic epistasis indicated that StkA functions downstream of T4P but upstream of the Dif proteins as a negative regulator of EPS production in M. xanthus.en
dc.description.sponsorshipNational Science Foundationen
dc.description.sponsorshipMCB-1417726en
dc.description.sponsorshipNational Institutes of Healthen
dc.description.sponsorshipGM071601en
dc.description.sponsorshipVirginia Tech. Fralin Life Science Instituteen
dc.description.sponsorshipVirginia Tech. Open Access Subvention Funden
dc.format.extent15 p.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMoak PL, Black WP, Wallace RA, Li Z, Yang Z. (2015) The Hsp70-like StkA functions between T4P and Dif signaling proteins as a negative regulator of exopolysaccharide in Myxococcus xanthus. PeerJ 3:e747 https://doi.org/10.7717/peerj.747en
dc.identifier.doihttps://doi.org/10.7717/peerj.747en
dc.identifier.issn2167-8359en
dc.identifier.pmid25674362en
dc.identifier.urihttp://hdl.handle.net/10919/64814en
dc.identifier.urlhttps://peerj.com/articles/747/en
dc.identifier.volume3en
dc.language.isoen_USen
dc.publisherPeerJen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderMoak, Pamela L.en
dc.rights.holderBlack, Wesley P.en
dc.rights.holderWallace, Regina A.en
dc.rights.holderLi, Zhuoen
dc.rights.holderYang, Zhaomien
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMyxococcus xanthusen
dc.subjectType IV pilus (T4P)en
dc.subjectDif pathwayen
dc.subjectExopolysaccharide (EPS)en
dc.subjectSocial motilityen
dc.subjectStkA/DnaK/Hsp70en
dc.titleThe Hsp70-like StkA functions between T4P and Dif signaling proteins as a negative regulator of exopolysaccharide in Myxococcus xanthusen
dc.title.serialPeerJen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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