Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores

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dc.contributor.authorKajtez, Jankoen
dc.contributor.authorSolomatina, Anastasiaen
dc.contributor.authorNovak, Majaen
dc.contributor.authorPolak, Brunoen
dc.contributor.authorVukusic, Krunoen
dc.contributor.authorRuediger, Jonasen
dc.contributor.authorCojoc, Gheorgheen
dc.contributor.authorMilas, Anaen
dc.contributor.authorSestak, Ivana Sumanovacen
dc.contributor.authorRisteski, Patriken
dc.contributor.authorTavano, Federicaen
dc.contributor.authorKlemm, Anna H.en
dc.contributor.authorRoscioli, Emanueleen
dc.contributor.authorWelburn, Julieen
dc.contributor.authorCimini, Danielaen
dc.contributor.authorGluncic, Matkoen
dc.contributor.authorPavin, Nenaden
dc.contributor.authorTolic, Iva M.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-02-08T14:44:19Zen
dc.date.available2019-02-08T14:44:19Zen
dc.date.issued2016-01en
dc.description.abstractDuring metaphase, forces on kinetochores are exerted by k-fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser-cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non-kinetochore microtubules, which we term 'bridging fibre', bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.en
dc.description.notesWe thank Ina Poser, Tony Hyman, Frank Bradke, Linda Wordeman and Mariola Chacon for cell lines and plasmids; Britta Schroth-Diez, Jan Peychl, Sebastian Bundschuh and Davide Accardi from the Light Microscopy Facility of MPI-CBG for support with microscopy; Petrina Delivani, Mariola Chacon, Titus Franzmann, Hannes Weisse, Emma Stepinac and other members of the Tolic laboratory for extensive help and advice; Ina Nusslein from the FACS Facility of MPI-CBG for assistance; Jan Brugues for discussions; Sophie Dumont for constructive comments on the manuscript; Ivana Saric for the drawings; Unity through Knowledge Fund (UKF), Human Frontier Science Programme (HFSP), German Research Foundation (DFG) and the European Research Council (ERC) for funding.en
dc.description.sponsorshipUnity through Knowledge Fund (UKF)en
dc.description.sponsorshipHuman Frontier Science Programme (HFSP)en
dc.description.sponsorshipGerman Research Foundation (DFG)en
dc.description.sponsorshipEuropean Research Council (ERC)en
dc.description.sponsorshipCancer Research UK [12840]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/ncomms10298en
dc.identifier.issn2041-1723en
dc.identifier.other10298en
dc.identifier.pmid26728792en
dc.identifier.urihttp://hdl.handle.net/10919/87540en
dc.identifier.volume7en
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleOverlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochoresen
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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