Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores

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dc.contributor.authorZahid, Osama K.en
dc.contributor.authorZhao, Boxuan Simenen
dc.contributor.authorHe, Chuanen
dc.contributor.authorHall, Adam R.en
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2019-01-23T14:27:28Zen
dc.date.available2019-01-23T14:27:28Zen
dc.date.issued2016-07-07en
dc.description.abstract5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA.en
dc.description.notesThe authors wish to thank the Howarth Lab (Oxford University) for supplying monovalent streptavidinand acknowledge services from the Wake Forest Cancer Genomics Shared Resource, supported by the Wake Forest Baptist Comprehensive Cancer Center's NCI Cancer Center Support Grant P30CA012197. This work was supported by NIH grant 1R21CA193067. O.K.Z. received support from the Sigma Xi Grants-in-Aid of research program (Grant ID No. 489441). A.R.H. acknowledges support from the 3 M Non-tenured Faculty Award program.en
dc.description.sponsorshipWake Forest Baptist Comprehensive Cancer Center's NCI Cancer Center [P30CA012197]; NIH [1R21CA193067]; Sigma Xi [489441]; 3 M Non-tenured Faculty Award programen
dc.format.extent6en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep29565en
dc.identifier.issn2045-2322en
dc.identifier.other29565en
dc.identifier.pmid27383905en
dc.identifier.urihttp://hdl.handle.net/10919/86846en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject5-hydroxymethylcytosineen
dc.subject5-formylcytosineen
dc.subject5-methylcytosineen
dc.subjectdifferentiationen
dc.subjectquantificationen
dc.subjectmicrornasen
dc.subjectproteinsen
dc.subjectbrainen
dc.titleQuantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanoporesen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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