Microbial composition of purified waters and implications for regrowth control in municipal water systems

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dc.contributor.authorProctor, Caitlin R.en
dc.contributor.authorEdwards, Marc A.en
dc.contributor.authorPruden, Amyen
dc.contributor.departmentCivil and Environmental Engineeringen
dc.date.accessed2016-03-17en
dc.date.accessioned2016-03-18T22:24:55Zen
dc.date.available2016-03-18T22:24:55Zen
dc.date.issued2015-08-25en
dc.description.abstractThe limits of water treatment to control microbial regrowth were examined using highly purified waters. Measurable microbial genetic material was detected in the product water in a survey of thirteen laboratory pure water systems. Illumina 16S rRNA gene amplicon sequencing revealed surprisingly diverse microbial assemblages, confirmed to be active in bioassays, with no direct relationship to quality or maintenance of the systems. With storage under both light and dark conditions, a 2-log increase in bacterial genetic markers was observed within 10 days, indicating viable oligotrophic communities despite rigorous treatment steps. With growth, microbial communities shifted concurrent with enrichment of Proteobacteria groups capable of nitrogen fixation (Bradyrhizobium) and H2 oxidation (Comamonadaceae). This study has implications not only for laboratory studies, which rely on highly purified waters, but also for municipal drinking water, which depends on treatment to reduce nutrients sufficiently to limit downstream regrowth of microorganisms.en
dc.description.notesContains supplementary information fileen
dc.description.notes2015 Royal Society of Chemistry Open Access Gold Articleen
dc.description.sponsorshipNational Science Foundation (U.S.)en
dc.description.sponsorshipAlfred P. Sloan Foundation. Microbiology of the Built Environment Programen
dc.description.sponsorshipVirginia Tech. Institute for Critical Technology and Applied Scienceen
dc.format.extent11 p.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationProctor, C. R., Edwards, M. A., & Pruden, A. (2015). Microbial composition of purified waters and implications for regrowth control in municipal water systems. Environmental Science: Water Research & Technology, 1(6), 882-892. doi:10.1039/C5EW00134Jen
dc.identifier.doihttps://doi.org/10.1039/C5EW00134Jen
dc.identifier.issn2053-1400en
dc.identifier.issue6en
dc.identifier.other2015_Proctor_Microbial_composition_of_purified.pdfen
dc.identifier.otherProctor_Microbial_composition_supp_file.pdfen
dc.identifier.otherCBET Award 1336650en
dc.identifier.urihttp://hdl.handle.net/10919/64961en
dc.identifier.volume1en
dc.language.isoenen
dc.publisherThe Royal Society of Chemistryen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.titleMicrobial composition of purified waters and implications for regrowth control in municipal water systemsen
dc.title.serialEnvironmental Science: Water Research & Technologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Scholarly Works, Civil and Environmental Engineering
Destination Area: Global Systems Science (GSS)