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An Engineering Analysis of the Stoichiometry of Autotrophic, Heterotrophic Bacterial Control of Ammonia-Nitrogen in Zero-Exchange Marine Shrimp Production Systems

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dc.contributor.authorEbeling, J. M.en
dc.contributor.authorTimmons, M. B.en
dc.contributor.authorBisogni, J. J.en
dc.contributor.editorSmith, Stephen Allenen
dc.date.accessioned2019-06-25T16:55:34Zen
dc.date.available2019-06-25T16:55:34Zen
dc.date.issued2009-06-01en
dc.description.abstractAfter dissolved oxygen, ammonia-nitrogen buildup from the metabolism of feed is usually the limiting factor to increasing production levels in intensive aquaculture systems. Currently, large fixed-cell bioreactors are the primary strategy used to control inorganic nitrogen in intensive recirculating systems. This option utilizes chemosynthetic autotrophic bacteria, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB). Zero-exchange nitrification management systems have been developed based on heterotrophic bacteria and promoted for the intensive production of marine shrimp and tilapia. In these systems, the heterotrophic bacterial growth is stimulated through the addition of an organic labile carbonaceous substrate. At high organic carbon to nitrogen (C/N) feed ratios, heterotrophic bacteria assimilate ammonia-nitrogen directly from the water, replacing the need for an external fixed film biofilter. As a result, build-up of suspended solids may become the second limiting factor after dissolved oxygen. This paper reviews two nitrogen conversion pathways used for the removal of ammonia-nitrogen in aquaculture systems; autotrophic bacterial conversion of ammonia-nitrogen to nitrate nitrogen, and heterotrophic bacterial conversion of ammonia-nitrogen directly to microbial biomass. The first part of this study reviews these two ammonia removal pathways, presents a set of balanced stoichiometric relationships, and discusses their impact on water quality. In addition, microbial growth energetics are used to characterize production of volatile and total suspended solids for autotrophic and heterotrophic systems. A critical verification of this work was that only a small fraction of the feed's carbon content is readily available to the heterotrophic bacteria. For example, feed containing 35% protein (350 g/kg feed) has only 109 g/kg feed of labile carbon. In the paper's second part, the results of a study on the impact C/N ratio on water quality is presented. In this experimental trial sufficient labile organic carbon in the form of sucrose (sugar) was added daily at 0%, 50%, and 100% of the system feeding rate to three prototype zero-exchange systems. The system was stocked with marine shrimp (Litopenaeus vannamei) at modest density (150 /m2) and water quality was measured daily. Significant differences were seen between the three strategies in the key water quality parameters of ammonia-nitrogen, nitrite-nitrogen, nitrate-nitrogen, pH, and alkalinity. The control (0%) system exhibited water quality characteristics of a mixed autotrophic/heterotrophic system while the other two systems receiving supplemental organic carbon (50% and 100%) showed water quality characteristics of pure heterotrophic systems.en
dc.format.extent27 pagesen
dc.format.extent788.87 KBen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationEbeling, J.M., Timmons, M.B. and Bisogni, J.J., 2009. An Engineering Analysis of the Stoichiometry of Autotrophic, Heterotrophic Bacterial Control of Ammonia-Nitrogen in Zero-Exchange Marine Shrimp Production Systems. International Journal of Recirculating Aquaculture, 10(1). DOI: http://doi.org/10.21061/ijra.v10i1.1336en
dc.identifier.doihttps://doi.org/10.21061/ijra.v10i1.1336en
dc.identifier.eissn2572-9160en
dc.identifier.issn1547-917Xen
dc.identifier.issue1en
dc.identifier.urihttp://hdl.handle.net/10919/90632en
dc.identifier.volume10en
dc.language.isoenen
dc.publisherCommercial Fish and Shellfish Technologies Program, Virginia Techen
dc.rightsIn Copyrighten
dc.rights.holderCommercial Fish and Shellfish Technologies Program, Virginia Techen
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectZero-exchange Systemsen
dc.subjectRecirculating Aquacultureen
dc.titleAn Engineering Analysis of the Stoichiometry of Autotrophic, Heterotrophic Bacterial Control of Ammonia-Nitrogen in Zero-Exchange Marine Shrimp Production Systemsen
dc.title.serialInternational Journal of Recirculating Aquacultureen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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