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Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana

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dc.contributor.authorRadakovits, Randoren
dc.contributor.authorJinkerson, Robert E.en
dc.contributor.authorFuerstenberg, Susan I.en
dc.contributor.authorTae, Hongseoken
dc.contributor.authorSettlage, Robert E.en
dc.contributor.authorBoore, Jeffrey L.en
dc.contributor.authorPosewitz, Matthew C.en
dc.date.accessioned2019-02-19T14:53:31Zen
dc.date.available2019-02-19T14:53:31Zen
dc.date.issued2012-02en
dc.description.abstractThe potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (similar to 29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.en
dc.description.notesWe would like to thank Anis Karimpour-Fard and Jason Shao for their help and advice. R.E.J. was supported by a Graduate Research Fellowship from the National Science Foundation. R.R. and this research were supported with funding provided by Conoco-Phillips through a grant to the Colorado Center for Biofuels and Biorefining (C2B2) and the Air Force Office of Scientific Research (FA9550-11-1-0211).en
dc.description.sponsorshipNational Science Foundationen
dc.description.sponsorshipConoco-Phillips [C2B2]en
dc.description.sponsorshipAir Force Office of Scientific Research [FA9550-11-1-0211]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/ncomms1688en
dc.identifier.issn2041-1723en
dc.identifier.other686en
dc.identifier.pmid22353717en
dc.identifier.urihttp://hdl.handle.net/10919/87720en
dc.identifier.volume3en
dc.language.isoen_USen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleDraft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditanaen
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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