Hypoxia-induced compression in the tracheal system of the tobacco hornworm caterpillar, Manduca sexta

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dc.contributorVirginia Techen
dc.contributor.authorGreenlee, Kendra J.en
dc.contributor.authorSocha, John J.en
dc.contributor.authorEubanks, Haleigh B.en
dc.contributor.authorPedersen, Paulen
dc.contributor.authorLee, Wah-Keaten
dc.contributor.authorKirkton, Scott D.en
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessed2014-02-04en
dc.date.accessioned2014-02-07T18:29:39Zen
dc.date.available2014-02-07T18:29:39Zen
dc.date.issued2013-06-01en
dc.description.abstractAbdominal pumping in caterpillars has only been documented during molting. Using synchrotron X-ray imaging in conjunction with high-speed flow-through respirometry, we show that Manduca sexta caterpillars cyclically contract their bodies in response to hypoxia, resulting in significant compressions of the tracheal system. Compression of tracheae induced by abdominal pumping drives external gas exchange, as evidenced by the high correlation between CO2 emission peaks and body movements. During abdominal pumping, both the compression frequency and fractional change in diameter of tracheae increased with body mass. However, abdominal pumping and tracheal compression were only observed in larger, older caterpillars (>0.2.g body mass), suggesting that this hypoxic response increases during ontogeny. The diameters of major tracheae in the thorax increased isometrically with body mass. However, tracheae in the head did not scale with mass, suggesting that there is a large safety margin for oxygen delivery in the head in the youngest animals. Together, these results highlight the need for more studies of tracheal system scaling and suggest that patterns of tracheal investment vary regionally in the body.en
dc.description.sponsorshipNational Science Foundation EPS-0447679, IOS-0953297, EFRI-0938047en
dc.description.sponsorshipNational Institutes of Health (NIH) National Center for Research Resources 2P20RR0l5566en
dc.description.sponsorshipInstitute for Critical Technology and Applied Science 118130en
dc.description.sponsorshipUS Department of Energy, Office of Science, Office of Basic Energy Sciences DE-AC02-06CH11357en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGreenlee, Kendra J.; Socha, John J.; Eubanks, Haleigh B.; et al., "Hypoxia-induced compression in the tracheal system of the tobacco hornworm caterpillar, Manduca sexta," J Exp Biol 216, 2293-2301 (2103); doi: 10.1242/_jeb.082479en
dc.identifier.doihttps://doi.org/10.1242/jeb.082479en
dc.identifier.issn0022-0949en
dc.identifier.urihttp://hdl.handle.net/10919/25337en
dc.identifier.urlhttp://jeb.biologists.org/content/216/12/2293.full.pdf+htmlen
dc.language.isoen_USen
dc.publisherCompany of Biologists Ltd.en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectInsecten
dc.subjectRespirationen
dc.subjectTracheaeen
dc.subjectVentilationen
dc.subjectCaterpillaren
dc.subjectFalse discovery rateen
dc.subjectSchistocerca-americanaen
dc.subjectRespiratory-functionen
dc.subjectGas exchangeen
dc.subjectBody-sizeen
dc.subjectOxygenen
dc.subjectGrasshoppersen
dc.subjectEcdysisen
dc.subjectVentilationen
dc.subjectResponsesen
dc.titleHypoxia-induced compression in the tracheal system of the tobacco hornworm caterpillar, Manduca sextaen
dc.title.serialJournal of Experimental Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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