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Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain

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dc.contributor.authorRossmeisl, John H. Jr.en
dc.contributor.authorGarcia, Paulo A.en
dc.contributor.authorRobertson, John L.en
dc.contributor.authorEllis, Thomas L.en
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.departmentSmall Animal Clinical Sciencesen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2016-11-04T18:34:36Zen
dc.date.available2016-11-04T18:34:36Zen
dc.date.issued2013-12-01en
dc.description.abstractThis study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation protocol with an additional dog receiving sham treatment. Animals were allowed to recover following N-TIRE ablation and the effects of treatment were monitored with clinical and magnetic resonance imaging examinations. Brains were subjected to histopathologic and ultrastructural assessment along with Bcl-2, caspase-3, and caspase-9 immunohistochemical staining following sacrifice 72 h post-treatment. Adverse clinical effects of N-TIRE were only observed in the dog treated at the upper energy tier. MRI and neuropathologic examinations indicated that N-TIRE ablation resulted in focal regions of severe cytoarchitectural and blood-brain-barrier disruption. Lesion size correlated to the intensity of the applied electrical field. N-TIRE-induced lesions were characterized by parenchymal necrosis and hemorrhage; however, large blood vessels were preserved. A transition zone containing parenchymal edema, perivascular inflammatory cuffs, and reactive gliosis was interspersed between the necrotic focus and normal neuropil. Apoptotic labeling indices were not different between the N-TIRE-treated and control brains. This study identified N-TIRE pulse parameters that can be used to safely create circumscribed foci of brain necrosis while selectively preserving major vascular structures.en
dc.description.sponsorshipThis work was supported in part by the Wallace Coulter and National Science Foundations (NSF-CBET 0933335). The authors thank Dr. Bernard Jortner, Dr. Elankumaran Subbiah, Ms. Jennifer Rudd, and Mrs. Barbara Wheeler for technical assistance with the ultrastructural and immunohistochemical studies. Angiodynamics, Inc. (USA) provided the NanoKnife pulse generator and electrodes used in this study.en
dc.description.versionPublished versionen
dc.format.extent433 - 440 (8) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.4142/jvs.2013.14.4.433en
dc.identifier.issn1229-845Xen
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/73377en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherKorean Society of Veterinary Scienceen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000328927400009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectScience & Technologyen
dc.subjectLife Sciences & Biomedicineen
dc.subjectVeterinary Sciencesen
dc.subjectVETERINARY SCIENCESen
dc.subjectcentral nervous systemen
dc.subjectdogen
dc.subjectirreversible electroporationen
dc.subjectneuropathologyen
dc.subjectTISSUE ABLATIONen
dc.subjectELECTROCHEMOTHERAPYen
dc.subjectULTRASOUNDen
dc.subjectTHERAPYen
dc.subjectSYSTEMen
dc.titlePathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brainen
dc.title.serialJournal of Veterinary Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Biomedical Engineering and Mechanicsen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Small Animal Clinical Sciencesen

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Scholarly Works, School of Biomedical Engineering and Sciences
Destination Area: Adaptive Brain and Behavior (ABB)
Scholarly Works, Small Animal Clinical Sciences