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High Resolution Respirometry of Heart Mitochondria in Healthy and Stressed States

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dc.contributor.authorWilson, Zachary T.en
dc.contributor.authorPerry, Justin B.en
dc.contributor.authorBrown, David A.en
dc.date.accessioned2018-08-15T12:50:05Zen
dc.date.available2018-08-15T12:50:05Zen
dc.date.issued2018-08en
dc.description.abstractHeart disease remains the leading cause of death globally, claiming the lives of nearly 10 million people in 2016. Current standard-of-care therapies for heart disease patients reduce energy demands on the heart but do not treat underlying deficits in cellular energy production. Cardiac mitochondria are primarily responsible for the production of energy in the heart, and targeting dysfunctional mitochondria represents a promising solution to improving the prognosis of heart disease patients. Increased production of reactive oxygen species in heart disease damages mitochondrial function, ultimately decreasing cardiac energy supply. Isolated mitochondria exposed to hydrogen peroxide serves as a heart disease model where the reactive oxygen species damage the respiratory chain, a series of complexes responsible for the actual production of energy. N-acetylcysteine (NAC) is a drug precursor to glutathione, an endogenous antioxidant which reduces reactive oxygen species. In this project, isolated mitochondria are treated with hydrogen peroxide with or without NAC. If NAC is capable of rescuing the respiratory rate, that would suggest that NAC restores mitochondrial energy production in pathological states. If successful, these data would be the first step in determining if incorporation of NAC into heart disease treatment plans could begin to better treat the number of one cause of morbidity/mortality on the planet.en
dc.description.notesUndergraduate research poster for research completed during the summer of 2018 as part of the Translational Obesity Undergraduate Research (TOUR) program.en
dc.description.sponsorshipNIH NHLBI RO1 HL123647en
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/84553en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectMitochondriaen
dc.subjectHeart diseaseen
dc.subjectOxygraph 2Ken
dc.subjectO2Ken
dc.subjectFree radicalsen
dc.subjectHydrogen peroxideen
dc.subjectRespiratory control ratioen
dc.subjectRCRen
dc.subjectN-acetylcysteineen
dc.subjectNACen
dc.subjectGlutathioneen
dc.titleHigh Resolution Respirometry of Heart Mitochondria in Healthy and Stressed Statesen
dc.typePosteren
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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