Phylogenomics of Xanthomonas field strains infecting pepper and tomato reveals diversity in effector repertoires and identifies determinants of host specificity

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dc.contributor.authorSchwartz, Allison R.en
dc.contributor.authorPotnist, Nehaen
dc.contributor.authorMilsina, Sujanen
dc.contributor.authorWilson, Marken
dc.contributor.authorPatane, Joseen
dc.contributor.authorMartins, Joaquim, Jr.en
dc.contributor.authorMinsavage, Gerald V.en
dc.contributor.authorDahlbeck, Douglasen
dc.contributor.authorAkhunova, Alinaen
dc.contributor.authorAlmeida, Nalvo F.en
dc.contributor.authorVallad, Gary E.en
dc.contributor.authorBarak, Jeri D.en
dc.contributor.authorWhite, Frank F.en
dc.contributor.authorMiller, Sally A.en
dc.contributor.authorRitchie, Daviden
dc.contributor.authorGoss, Ericaen
dc.contributor.authorBart, Rebecca S.en
dc.contributor.authorSetubal, Joao C.en
dc.contributor.authorJones, Jeffrey B.en
dc.contributor.authorStaskawicz, Brian J.en
dc.date.accessioned2019-03-20T12:48:11Zen
dc.date.available2019-03-20T12:48:11Zen
dc.date.issued2015-06-03en
dc.description.abstractBacterial spot disease of pepper and tomato is caused by four distinct Xanthomonas species and is a severely limiting factor on fruit yield in these crops. The genetic diversity and the type Ill effector repertoires of a large sampling of field strains for this disease have yet to be explored on a genomic scale, limiting our understanding of pathogen evolution in an agricultural setting. Genomes of 67 Xanthomonas euvesicatoria (Xe), Xanthomonas perforans (Xp), and Xanthomonas gardneri (Kg) strains isolated from diseased pepper and tomato fields in the southeastern and midwestern United States were sequenced in order to determine the genetic diversity in field strains. Type Ill effector repertoires were computationally predicted for each strain, and multiple methods of constructing phylogenies were employed to understand better the genetic relationship of strains in the collection. A division in the Xp population was detected based on core genome phylogeny, supporting a model whereby the host-range expansion of Xp field strains on pepper is due, in part, to a loss of the effector AvrBsT. Xp-host compatibility was further studied with the observation that a double deletion of AvrBsT and XopQ allows a host range expansion for Nicotiana benthamiana. Extensive sampling of field strains and an improved understanding of effector content will aid in efforts to design disease resistance strategies targeted against highly conserved core effectors.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2015.00535en
dc.identifier.issn1664-302Xen
dc.identifier.other535en
dc.identifier.pmid26089818en
dc.identifier.urihttp://hdl.handle.net/10919/88497en
dc.identifier.volume6en
dc.language.isoen_USen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titlePhylogenomics of Xanthomonas field strains infecting pepper and tomato reveals diversity in effector repertoires and identifies determinants of host specificityen
dc.title.serialFrontiers in Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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