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TALEN-Mediated Modification of the Bovine Genome for Large-Scale Production of Human Serum Albumin

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dc.contributor.authorMoghaddassi, Shaidaen
dc.contributor.authorEyestone, Willen
dc.contributor.authorBishop, Colin E.en
dc.date.accessioned2018-10-10T19:22:27Zen
dc.date.available2018-10-10T19:22:27Zen
dc.date.issued2014-02-21en
dc.description.abstractAs an initial step towards creating genetically modified cattle as a biopharming source of recombinant human serum albumin (rHSA), we report modification of the bovine albumin (bA) locus by transcription activator-like effector nuclease (TALEN)-stimulated homology-directed repair (HDR). Pedigreed bovine fibroblasts were co-transfected with TALENs and an 11.5-kb human serum albumin (HSA) minigene donor construct, designed to simultaneously disrupt and replace bovine serum albumin (BSA) expression with controlled rHSA expression in both the liver and the milk. Targeted integration of the HSA minigene was confirmed in transfected fibroblasts at a frequency of approximately 11% and transgenic bovine embryos were produced from targeted fibroblasts using somatic cell nuclear transfer (SCNT). The research delineated here lays the foundation for the future generation of transgenic rHSA cattle with the potential to provide a large-scale, reliable, and quality-controlled source of rHSA.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0089631en
dc.identifier.eissn1932-6203en
dc.identifier.issue2en
dc.identifier.othere89631en
dc.identifier.pmid24586924en
dc.identifier.urihttp://hdl.handle.net/10919/85342en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleTALEN-Mediated Modification of the Bovine Genome for Large-Scale Production of Human Serum Albuminen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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