DNA methylation Landscape of body size variation in sheep

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dc.contributor.authorCao, Jiaxueen
dc.contributor.authorWei, Caihongen
dc.contributor.authorLiu, Dongmingen
dc.contributor.authorWang, Huihuaen
dc.contributor.authorWu, Mingmingen
dc.contributor.authorXie, Zhiyuanen
dc.contributor.authorCapellini, Terence D.en
dc.contributor.authorZhang, Lien
dc.contributor.authorZhao, Fupingen
dc.contributor.authorLi, Lien
dc.contributor.authorZhong, Taoen
dc.contributor.authorWang, Linjieen
dc.contributor.authorLu, Jianen
dc.contributor.authorLiu, Ruizaoen
dc.contributor.authorZhang, Shifangen
dc.contributor.authorDu, Yongfeien
dc.contributor.authorZhang, Hongpingen
dc.contributor.authorDu, Lixinen
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2019-01-24T18:24:47Zen
dc.date.available2019-01-24T18:24:47Zen
dc.date.issued2015-10-16en
dc.description.abstractSub-populations of Chinese Mongolian sheep exhibit significant variance in body mass. In the present study, we sequenced the whole genome DNA methylation in these breeds to detect whether DNA methylation plays a role in determining the body mass of sheep by Methylated DNA immunoprecipitation - sequencing method. A high quality methylation map of Chinese Mongolian sheep was obtained in this study. We identified 399 different methylated regions located in 93 human orthologs, which were previously reported as body size related genes in human genome-wide association studies. We tested three regions in LTBP1, and DNA methylation of two CpG sites showed significant correlation with its RNA expression. Additionally, a particular set of differentially methylated windows enriched in the "development process" (GO: 0032502) was identified as potential candidates for association with body mass variation. Next, we validated small part of these windows in 5 genes; DNA methylation of SMAD1, TSC1 and AKT1 showed significant difference across breeds, and six CpG were significantly correlated with RNA expression. Interestingly, two CpG sites showed significant correlation with TSC1 protein expression. This study provides a thorough understanding of body size variation in sheep from an epigenetic perspective.en
dc.description.notesWe are grateful to Qing Ma (Ningxia Hui Autonomous Region) for animal sampling. We also thank Long Jin, Mingzhou Li (Sichuan Agricultural University), Xinlei Zhou, Xiaoning Zhang, Jiashen Liu and Guobin Lu (CAAS) for their generous assistance. We acknowledge Yu Zhang (Bio Miao Biological Technology, Beijing, Co., Ltd.). This research was supported by the National Key Technology R&D Program of China (2011BAD28B05-2), and the National Meat Sheep System Specialist Program.en
dc.description.sponsorshipNational Key Technology R&D Program of China [2011BAD28B05-2]; National Meat Sheep System Specialist Programen
dc.format.extent13en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep13950en
dc.identifier.issn2045-2322en
dc.identifier.other13950en
dc.identifier.pmid26472088en
dc.identifier.urihttp://hdl.handle.net/10919/86880en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcell-growthen
dc.subjectenvironmental epigeneticsen
dc.subjectdrosophila homologen
dc.subjectbone-formationen
dc.subjectorgan sizeen
dc.subjecttsc1en
dc.subjectpathwayen
dc.subjectfibroblastsen
dc.subjectexpressionen
dc.subjectstabilityen
dc.titleDNA methylation Landscape of body size variation in sheepen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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