Eubacterial SpoVG Homologs Constitute a New Family of Site-Specific DNA-Binding Proteins
| dc.contributor.author | Jutras, Brandon L. | en |
| dc.contributor.author | Chenail, Alicia M. | en |
| dc.contributor.author | Rowland, Christi L. | en |
| dc.contributor.author | Carroll, Dustin W. | en |
| dc.contributor.author | Miller, M. Clarke | en |
| dc.contributor.author | Bykowski, Tomasz | en |
| dc.contributor.author | Stevenson, Brian | en |
| dc.contributor.department | Biochemistry | en |
| dc.date.accessioned | 2019-02-13T19:17:23Z | en |
| dc.date.available | 2019-02-13T19:17:23Z | en |
| dc.date.issued | 2013-06-20 | en |
| dc.description.abstract | A site-specific DNA-binding protein was purified from Borrelia burgdorferi cytoplasmic extracts, and determined to be a member of the highly conserved SpoVG family. This is the first time a function has been attributed to any of these ubiquitous bacterial proteins. Further investigations into SpoVG orthologues indicated that the Staphylococcus aureus protein also binds DNA, but interacts preferentially with a distinct nucleic acid sequence. Site-directed mutagenesis and domain swapping between the S. aureus and B. burgdorferi proteins identified that a 6-residue stretch of the SpoVG a-helix contributes to DNA sequence specificity. Two additional, highly conserved amino acid residues on an adjacent b-sheet are essential for DNA-binding, apparently by contacts with the DNA phosphate backbone. Results of these studies thus identified a novel family of bacterial DNA-binding proteins, developed a model of SpoVG-DNA interactions, and provide direction for future functional studies on these wide-spread proteins. | en |
| dc.description.sponsorship | National Research Fund for Tick-Borne Diseases | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1371/journal.pone.0066683 | en |
| dc.identifier.issue | 6 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/87574 | en |
| dc.identifier.volume | 8 | en |
| dc.language.iso | en | en |
| dc.publisher | PLOS | en |
| dc.rights | Creative Commons Attribution 3.0 United States | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | en |
| dc.title | Eubacterial SpoVG Homologs Constitute a New Family of Site-Specific DNA-Binding Proteins | en |
| dc.title.serial | PLOS One | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |