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Comparative nutritional and chemical phenome of Clostridium difficile isolates determined using phenotype microarrays

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dc.contributor.authorScaria, Joyen
dc.contributor.authorChen, Jenn-Weien
dc.contributor.authorUseh, Nicodemusen
dc.contributor.authorHe, Hongxuanen
dc.contributor.authorMcDonough, Sean P.en
dc.contributor.authorMao, Chunhongen
dc.contributor.authorSobral, Brunoen
dc.contributor.authorChang, Yung-Fuen
dc.date.accessioned2019-08-21T17:43:58Zen
dc.date.available2019-08-21T17:43:58Zen
dc.date.issued2014-10en
dc.description.abstractObjectives: Clostridium difficile infection (CDI) is the leading cause of infectious diarrhea in North America and Europe. The risk of CDI increases significantly in the case where antimicrobial treatment reduces the number of competing bacteria in the gut, thus leading to the increased availability of nutrients and loss of colonization resistance. The objective of this study was to determine comprehensive nutritional utilization and the chemical sensitivity profile of historic and newer C. difficile isolates and to examine the possible role of the phenotype diversity in C. difficile virulence. Methods: Phenotype microarrays (PMs) were used to elucidate the complete nutritional and chemical sensitivity profile of six C. difficile isolates. Results: Of the 760 nutrient sources tested, 285 compounds were utilized by at least one strain. Among the C. difficile isolates compared, R20291, a recent hypervirulent outbreak-associated strain, appears to have an expanded nutrient utilization profile when compared to all other strains. Conclusions: The expanded nutritional utilization profile of some newer C. difficile strains could be one of the reasons for infections in patients who are not exposed to the hospital environment or not undergoing antibiotic treatment. This nutritional profile could be used to design tube feeding formulas that reduce the risk of CDI.en
dc.description.notesThis work was supported in part by a subcontract from Virginia Tech (project No. 62078/A001), which has been funded by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, and Department of Health and Human Services, under Contract No. HHSN272200900040C, and a grant from the USDA Animal Health and Disease Research Program (NYCV-478820).en
dc.description.sponsorshipVirginia Tech [62078/A001]; National Institute of Allergy and Infectious Diseases; National Institutes of Health, and Department of Health and Human Services [HHSN272200900040C]; USDA Animal Health and Disease Research Program [NYCV-478820]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.ijid.2014.06.018en
dc.identifier.eissn1878-3511en
dc.identifier.issn1201-9712en
dc.identifier.pmid25130165en
dc.identifier.urihttp://hdl.handle.net/10919/93200en
dc.language.isoenen
dc.publisherInternational Society for Infectious Diseasesen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectClostridium difficileen
dc.subjectPhenotype microarrayen
dc.subjectPhenomeen
dc.subjectPhenotypeen
dc.subjectMetabolismen
dc.titleComparative nutritional and chemical phenome of Clostridium difficile isolates determined using phenotype microarraysen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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