Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments

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dc.contributor.authorMercadal, Borjaen
dc.contributor.authorBeitel-White, Natalieen
dc.contributor.authorAycock, Kenneth N.en
dc.contributor.authorCastellvi, Quimen
dc.contributor.authorDavalos, Rafael V.en
dc.contributor.authorIvorra, Antonien
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessioned2021-01-07T18:13:53Zen
dc.date.available2021-01-07T18:13:53Zen
dc.date.issued2020-02-05en
dc.description.abstractHigh-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.en
dc.description.notesThe authors would like to thank Dr. Elisa M. Wasson for her help in the image analysis of 3D cell cultures. This work was supported by the Ministry of Economy and Competitiveness of Spain (Grant No. TEC2014-52383-C3-2-R), the Pancreatic Cancer Action Network (Grant No. 16-65-IANN) and Cures Within Reach (Grant No. PUJFSANY). Antoni Ivorra gratefully acknowledges financial support by ICREA under the ICREA Academia programme.en
dc.description.sponsorshipMinistry of Economy and Competitiveness of Spain [TEC2014-52383-C3-2-R]; Pancreatic Cancer Action Network [16-65-IANN]; Cures Within Reach [PUJFSANY]; ICREA under the ICREA Academia programmeen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s10439-020-02462-8en
dc.identifier.eissn1573-9686en
dc.identifier.issn0090-6964en
dc.identifier.issue5en
dc.identifier.pmid32026232en
dc.identifier.urihttp://hdl.handle.net/10919/101775en
dc.identifier.volume48en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectIrreversible electroporationen
dc.subjectHigh-frequency irreversible electroporationen
dc.subjectMembrane permeabilityen
dc.subjectCaspase 3/7en
dc.subjectBipolar pulsesen
dc.titleDynamics of Cell Death After Conventional IRE and H-FIRE Treatmentsen
dc.title.serialAnnals of Biomedical Engineeringen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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