Immunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Disease

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dc.contributor.authorGhavami, Shaghayegh Baradaranen
dc.contributor.authorYadegar, Abbasen
dc.contributor.authorAghdaei, Hamid Asadzadehen
dc.contributor.authorSorrentino, Darioen
dc.contributor.authorFarmani, Maryamen
dc.contributor.authorMir, Adil Shamimen
dc.contributor.authorAzimirad, Masoumehen
dc.contributor.authorBalaii, Hediehen
dc.contributor.authorShahrokh, Shabnamen
dc.contributor.authorZali, Mohammad Rezaen
dc.contributor.departmentVirginia Tech Carilion School of Medicineen
dc.date.accessioned2021-02-09T14:10:49Zen
dc.date.available2021-02-09T14:10:49Zen
dc.date.issued2020-09en
dc.description.abstractIn inflammatory bowel diseases (IBD), the therapeutic benefit and mucosal healing from specific probiotics may relate to the modulation of dendritic cells (DCs). Herein, we assessed the immunomodulatory effects of four probiotic strains includingLactobacillus salivarius,Bifidobacterium bifidum,Bacillus coagulansandBacillus subtilisnatto on the expression of co-stimulatory molecules, cytokine production and gene expression of signal-transducing receptors in DCs from IBD patients. Human monocyte-derived DCs from IBD patients and healthy controls were exposed to four probiotic strains. The expression of co-stimulatory molecules was assessed and supernatants were analyzed for anti-inflammatory cytokines. The gene expression of toll-like receptors (TLRs), IL-12p40 and integrin alpha v beta 8 were also analyzed. CD80 and CD86 were induced by most probiotic strains in ulcerative colitis (UC) patients whereas onlyB. bifiduminduced CD80 and CD86 expression in Crohn's disease (CD) patients. IL-10 and TGF-beta production was increased in a dose-independent manner while TLR expression was decreased by all probiotic bacteria exceptB. bifidumin DCs from UC patients. TLR-4 and TLR-9 expression was significantly downregulated while integrin ss8 was significantly increased in the DCs from CD patients. IL-12p40 expression was only significantly downregulated in DCs from CD patients. Our findings point to the general beneficial effects of probiotics in DC immunomodulation and indicate that probiotic bacteria favorably modulate the expression of co-stimulatory molecules, proinflammatory cytokines and TLRs in DCs from IBD patients.en
dc.description.notesThis research was funded by Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran, research grant No. RIGLD 975.en
dc.description.sponsorshipResearch Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran [RIGLD 975]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/ijms21176266en
dc.identifier.eissn1422-0067en
dc.identifier.issue17en
dc.identifier.other6266en
dc.identifier.pmid32872480en
dc.identifier.urihttp://hdl.handle.net/10919/102317en
dc.identifier.volume21en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectinflammatory bowel diseasesen
dc.subjectCrohn's diseaseen
dc.subjectulcerative colitisen
dc.subjectdendritic cellsen
dc.subjectimmune toleranceen
dc.subjectprobiotic bacteriaen
dc.subjectanti-inflammatory responseen
dc.titleImmunomodulation and Generation of Tolerogenic Dendritic Cells by Probiotic Bacteria in Patients with Inflammatory Bowel Diseaseen
dc.title.serialInternational Journal of Molecular Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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