Microfluidic systems and methods for chromatin immunoprecipitation (ChIP)
| dc.contributor.assignee | Virginia Tech Intellectual Properties, Inc. | en |
| dc.contributor.department | Chemical Engineering | en |
| dc.contributor.inventor | Lu, Chang | en |
| dc.contributor.inventor | Cao, Zhenning | en |
| dc.date.accessed | 2019-02-15 | en |
| dc.date.accessioned | 2019-02-26T22:23:57Z | en |
| dc.date.available | 2019-02-26T22:23:57Z | en |
| dc.date.filed | 2014-10-10 | en |
| dc.date.issued | 2017-08-15 | en |
| dc.description.abstract | An integrated microfluidic chromatin immunoprecipitation assay dramatically improves the collection efficiency of ChIP DNA from cells. Immunoprecipitation of chromatin fragments is conducted in a microfluidic chamber with a large fraction of its volume (e.g., ˜15-40%) occupied by magnetic immunoprecipitation (IP) beads. Oscillating washing of the beads, enabled by, e.g., solenoid valves (controlled by a computer) and high pressure attached to both ends of the microfluidic chamber, effectively removes unbound chromatin and produces high-quality ChIP DNA. ChIP DNA produced by an example device generates excellent results in the subsequent DNA library preparation. The ChIP-seq (i.e., ChIP followed by next-generation sequencing) results match very well with public data generated using much larger cell sample sizes and a conventional approach. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.applicationnumber | 14511422 | en |
| dc.identifier.patentnumber | 9732377 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/87816 | en |
| dc.identifier.url | http://pimg-fpiw.uspto.gov/fdd/77/323/097/0.pdf | en |
| dc.language.iso | en_US | en |
| dc.publisher | United States Patent and Trademark Office | en |
| dc.subject.cpc | B01L3/50276 | en |
| dc.subject.cpc | B01L2200/0668 | en |
| dc.subject.cpc | B01L2300/0816 | en |
| dc.subject.cpc | B01L2300/0877 | en |
| dc.subject.cpc | B01L2400/043 | en |
| dc.subject.cpc | B01L2400/0487 | en |
| dc.subject.cpc | C12Q1/6806 | en |
| dc.subject.cpc | C12Q2522/10 | en |
| dc.subject.cpc | C12Q2565/629 | en |
| dc.subject.cpc | G01N33/54326 | en |
| dc.subject.cpc | G01N33/6875 | en |
| dc.title | Microfluidic systems and methods for chromatin immunoprecipitation (ChIP) | en |
| dc.type | Patent | en |
| dc.type.dcmitype | Text | en |
| dc.type.patenttype | utility | en |
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