Rapid detection of Escherichia coli in field based potable water applications
| dc.contributor.author | Thayagan, Sathieseelan | en |
| dc.contributor.committeechair | Strawn, Laura K. | en |
| dc.contributor.committeemember | Strawn, Laura K. | en |
| dc.contributor.committeemember | Eifert, Joseph D. | en |
| dc.contributor.committeemember | Williams, Robert C. | en |
| dc.contributor.department | Food Science and Technology | en |
| dc.date.accessioned | 2018-04-13T20:41:06Z | en |
| dc.date.available | 2018-04-13T20:41:06Z | en |
| dc.date.issued | 2018-03-12 | en |
| dc.description.abstract | E.coli 0157:H7 is one of the major bacterial diseases transmitted through drinking water and is a major contaminant on leafy greens. Results from current traditional plating methods including P-A analysis is time consuming and this slow process is an hinderance as a preventive control for food-borne outbreaks. In this experiment a fluorescence immonoassay outlined in fig. 7, is proposed for detecting E.coli 0157:H7 in water with a moderate amount of preparatory steps and less than 5 minutes for the detection of this pathogen. In examining the relationship between E.coli 0157: H7 CFU/0.1mL to fluorescence intensity, our model data evaluated with Minitab 16, revealed a P(0.001)= 0, and F=271.56 demonstrating a stong relationship of E.coli 0157:H7 detection with this immunoassay. E.aerogenes the negative contol for the immunoassay revealed a P(0.001)=0.677 and F=0.31, validating the specificity of the model. E.coli 0157:H7 was detected at 1 CFU/0.1mL with the proposed model and least three sigma (3σ) levels above the baseline for the control blank, thereby showing ≥ 99.7% probability of locating E.coli 0157:H7 when compared to the experiment control. | en |
| dc.description.degree | MALS | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/82831 | en |
| dc.language.iso | en | en |
| dc.publisher | Virginia Tech | en |
| dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 United States | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | en |
| dc.subject.cabt | Qdot, streptavidin, E.coli 0157:H7, Escherichia coli, fluorescence | en |
| dc.title | Rapid detection of Escherichia coli in field based potable water applications | en |
| dc.type | Report | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Food Safety and Biosecurity | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | masters | en |
| thesis.degree.name | Master of Agricultural and Life Sciences | en |