Cytotoxicity and Cellular Uptake of Cellulose Nanocrystals
| dc.contributor.author | Dong, Shuping | en |
| dc.contributor.author | Hirani, Anjali A. | en |
| dc.contributor.author | Colacino, Katelyn R. | en |
| dc.contributor.author | Lee, Yong Woo | en |
| dc.contributor.author | Roman, Maren | en |
| dc.contributor.department | Sustainable Biomaterials | en |
| dc.date.accessioned | 2015-03-04T14:42:47Z | en |
| dc.date.available | 2015-03-04T14:42:47Z | en |
| dc.date.issued | 2012-09-21 | en |
| dc.description.abstract | There is growing evidence that filamentous nanoparticles offer advantages over spherical ones in drug delivery applications. The purpose of this study was to assess the potential of rod-like, plant-derived cellulose nanocrystals (CNCs) for nanomedical uses. Besides a nonspherical morphology, their facile bioconjugation, surface hydrophilicity and small size render CNCs promising drug carriers. The cytotoxicity of CNCs against nine different cell lines (HBMEC, bEnd.3, RAW 264.7, MCF-10A, MDA-MB-231, MDA-MB-468, KB, PC-3 and C6) was determined by MTT and LDH assay. CNCs showed no cytotoxic effects against any of these cell lines in the concentration range and exposure time studied (0–50 µg/mL and 48 h, respectively). Cellular uptake of fluorescein-50 - isothiocyanate-labeled CNCs by these cell lines, quantified with a fluorescence microplate reader, was minimal. The lack of cytotoxicity and the low nonspecific cellular uptake support our hypothesis that CNCs are good candidates for nanomedical applications. | en |
| dc.description.sponsorship | National Research Initiative of the USDA Cooperative State Research, Education and Extension Service - Grant 2005-35504-16088 | en |
| dc.description.sponsorship | National Science Foundation - Grants CHE-0724126 and DMR-0907567 | en |
| dc.description.sponsorship | Institute for Critical Technology and Applied Science | en |
| dc.description.sponsorship | Macromolecules and Interfaces Institute and Omnova Solutions, Inc. | en |
| dc.identifier.citation | Dong, S., Hirani, A. A., Colacino, K. R., Lee, Y. W., & Roman, M. (2012). Cytotoxicity and Cellular Uptake of Cellulose Nanocrystals. Nano LIFE, 2(3). doi: 10.1142/S1793984412410061 | en |
| dc.identifier.doi | https://doi.org/10.1142/S1793984412410061 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/51594 | en |
| dc.language.iso | en_US | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject | Nanoparticles | en |
| dc.subject | drug carriers | en |
| dc.subject | FITC conjugation | en |
| dc.subject | nanomedicine | en |
| dc.subject | animal cell cultures | en |
| dc.title | Cytotoxicity and Cellular Uptake of Cellulose Nanocrystals | en |
| dc.type | Article - Refereed | en |