Cytotoxicity and Cellular Uptake of Cellulose Nanocrystals

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dc.contributor.authorDong, Shupingen
dc.contributor.authorHirani, Anjali A.en
dc.contributor.authorColacino, Katelyn R.en
dc.contributor.authorLee, Yong Wooen
dc.contributor.authorRoman, Marenen
dc.contributor.departmentSustainable Biomaterialsen
dc.date.accessioned2015-03-04T14:42:47Zen
dc.date.available2015-03-04T14:42:47Zen
dc.date.issued2012-09-21en
dc.description.abstractThere is growing evidence that filamentous nanoparticles offer advantages over spherical ones in drug delivery applications. The purpose of this study was to assess the potential of rod-like, plant-derived cellulose nanocrystals (CNCs) for nanomedical uses. Besides a nonspherical morphology, their facile bioconjugation, surface hydrophilicity and small size render CNCs promising drug carriers. The cytotoxicity of CNCs against nine different cell lines (HBMEC, bEnd.3, RAW 264.7, MCF-10A, MDA-MB-231, MDA-MB-468, KB, PC-3 and C6) was determined by MTT and LDH assay. CNCs showed no cytotoxic effects against any of these cell lines in the concentration range and exposure time studied (0–50 µg/mL and 48 h, respectively). Cellular uptake of fluorescein-50 - isothiocyanate-labeled CNCs by these cell lines, quantified with a fluorescence microplate reader, was minimal. The lack of cytotoxicity and the low nonspecific cellular uptake support our hypothesis that CNCs are good candidates for nanomedical applications.en
dc.description.sponsorshipNational Research Initiative of the USDA Cooperative State Research, Education and Extension Service - Grant 2005-35504-16088en
dc.description.sponsorshipNational Science Foundation - Grants CHE-0724126 and DMR-0907567en
dc.description.sponsorshipInstitute for Critical Technology and Applied Scienceen
dc.description.sponsorshipMacromolecules and Interfaces Institute and Omnova Solutions, Inc.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDong, S., Hirani, A. A., Colacino, K. R., Lee, Y. W., & Roman, M. (2012). Cytotoxicity and Cellular Uptake of Cellulose Nanocrystals. Nano LIFE, 2(3). doi: 10.1142/S1793984412410061en
dc.identifier.doihttps://doi.org/10.1142/S1793984412410061en
dc.identifier.urihttp://hdl.handle.net/10919/51594en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectNanoparticlesen
dc.subjectdrug carriersen
dc.subjectFITC conjugationen
dc.subjectnanomedicineen
dc.subjectanimal cell culturesen
dc.titleCytotoxicity and Cellular Uptake of Cellulose Nanocrystalsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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