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Mechanical properties and consequences of stereocilia and extracellular links in vestibular hair bundles

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dc.contributorVirginia Techen
dc.contributor.authorNam, Jong-Hoonen
dc.contributor.authorCotton, John R.en
dc.contributor.authorPeterson, Ellengene H.en
dc.contributor.authorGrant, John Wallaceen
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessed2014-02-05en
dc.date.accessioned2014-02-26T19:10:06Zen
dc.date.available2014-02-26T19:10:06Zen
dc.date.issued2006-04-01en
dc.description.abstractAlthough knowledge of the. ne structure of vestibular hair bundles is increasing, the mechanical properties and functional significance of those structures remain unclear. In 2004, Bashtanov and colleagues reported the contribution of different extracellular links to bundle stiffness. We simulated Bashtanov's experimental protocol using a three-dimensional finite element bundle model with geometry measured from a typical striolar hair cell. Unlike any previous models, we separately consider two types of horizontal links: shaft links and upper lateral links. Our most important results are as follows. First, we identified the material properties required to match Bashtanov's experiment: stereocilia Young's modulus of 0.74 GPa, tip link assembly (gating spring) stiffness of 5300 pN/μm, and the combined stiffness of shaft links binding two adjacent stereocilia of 750 similar to 2250 pN/μm. Second, we conclude that upper lateral links are likely to have nonlinear mechanical properties: they have minimal stiffness during small bundle deformations but stiffen as the bundle deflects further. Third, we estimated the stiffness of the gating spring based on our realistic three-dimensional bundle model rather than a conventional model relying on the parallel arrangement assumption. Our predicted stiffness of the gating spring was greater than the previous estimation.en
dc.description.sponsorshipNIH DC05063en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationNam, JH; Cotton, JR; Peterson, EH; et al. "Mechanical properties and consequences of stereocilia and extracellular links in vestibular hair bundles," Biophysical Journal 90(8), 2786-2795 (2006); doi: 10.1529/biophysj.105.066027en
dc.identifier.doihttps://doi.org/10.1529/biophysj.105.066027en
dc.identifier.issn0006-3495en
dc.identifier.urihttp://hdl.handle.net/10919/25780en
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006349506724602en
dc.language.isoen_USen
dc.publisherCELL PRESSen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectTip linksen
dc.subjectInner-earen
dc.subjectMechanoelectrical transductionen
dc.subjectComputationalen
dc.subjectModelsen
dc.subjectCalcium chelationen
dc.subjectKinocilial linksen
dc.subjectActin-filamentsen
dc.subjectCiliaryen
dc.subjectBundlesen
dc.subjectCuticular plateen
dc.subjectCell-surfaceen
dc.titleMechanical properties and consequences of stereocilia and extracellular links in vestibular hair bundlesen
dc.title.serialBiophysical Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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