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Specific labeling of synaptic schwann cells reveals unique cellular and molecular features

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dc.contributor.authorCastro, Ryan W.en
dc.contributor.authorTaetzsch, Thomasen
dc.contributor.authorVaughan, Sydney K.en
dc.contributor.authorGodbe, Kerilynen
dc.contributor.authorChappell, John C.en
dc.contributor.authorSettlage, Robert E.en
dc.contributor.authorValdez, Gregorioen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentAdvanced Research Computingen
dc.date.accessioned2020-07-31T13:43:48Zen
dc.date.available2020-07-31T13:43:48Zen
dc.date.issued2020-06-25en
dc.description.abstractPerisynaptic Schwann cells (PSCs) are specialized, non-myelinating, synaptic glia of the neuromuscular junction (NMJ), that participate in synapse development, function, maintenance, and repair. The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. This limited approach has precluded our ability to isolate and genetically manipulate PSCs in a cell specific manner. We have identified neuron-glia antigen 2 (NG2) as a unique molecular marker of S100 beta+ PSCs in skeletal muscle. NG2 is expressed in Schwann cells already associated with the NMJ, indicating that it is a marker of differentiated PSCs. Using a newly generated transgenic mouse in which PSCs are specifically labeled, we show that PSCs have a unique molecular signature that includes genes known to play critical roles in PSCs and synapses. These findings will serve as a springboard for revealing drivers of PSC differentiation and function.en
dc.description.notesNational Institutes of Health R01AG055545 Gregorio Valdez; National Institutes of Health R56AG051501 Gregorio Valdez; National Institutes of Health R21NS106313 Gregorio Valdez; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.en
dc.description.sponsorshipNational Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01AG055545, R56AG051501, R21NS106313]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.7554/eLife.56935en
dc.identifier.issn2050-084Xen
dc.identifier.othere56935en
dc.identifier.pmid32584256en
dc.identifier.urihttp://hdl.handle.net/10919/99458en
dc.identifier.volume9en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSpecific labeling of synaptic schwann cells reveals unique cellular and molecular featuresen
dc.title.serialeLifeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Advanced Research Computing
Destination Area: Adaptive Brain and Behavior (ABB)