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Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth

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dc.contributor.authorCastillo, Hugoen
dc.contributor.authorLi, Xiaopingen
dc.contributor.authorSmith, Geoffrey B.en
dc.date.accessioned2021-11-23T14:29:30Zen
dc.date.available2021-11-23T14:29:30Zen
dc.date.issued2021-05-06en
dc.description.abstractIonizing radiation is a major environmental variable for cells on Earth, and so organisms have adapted to either prevent or to repair damages caused by it, primarily from the appearance and accumulation of reactive oxygen species (ROS). In this study, we measured the differential gene expression in Deinococcus radiodurans UWO298 cultures deprived of background ionizing radiation (IR) while growing 605 m underground at the Waste Isolation Pilot Plant (WIPP), reducing the dose rate from 72.1 to 0.9 nGy h(-1) from control to treatment, respectively. This reduction in IR dose rate delayed the entry into the exponential phase of the IR-shielded cultures, resulting in a lower biomass accumulation for the duration of the experiment. The RNASeq-based transcriptome analysis showed the differential expression of 0.2 and 2.7% of the D. radiodurans genome after 24 and 34 h of growth in liquid culture, respectively. Gene expression regulation after 34 h was characterized by the downregulation of genes involved in folding newly synthesized and denatured/misfolded proteins, in the assimilation of nitrogen for amino acid synthesis and in the control of copper transport and homeostasis to prevent oxidative stress. We also observed the upregulation of genes coding for proteins with transport and cell wall assembly roles. These results show that D. radiodurans is sensitive to the absence of background levels of ionizing radiation and suggest that its transcriptional response is insufficient to maintain optimal growth.en
dc.description.notesThese results are based upon work supported by the United States Department of Energy (DOE) Office of Environmental Management under award number DE-EM0002423. This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government or any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe on privately owned rights. Reference herein to any specific commercial product, process, or service by trade names, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement. The views and the opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.en
dc.description.sponsorshipUnited States Department of Energy (DOE) Office of Environmental ManagementUnited States Department of Energy (DOE) [DE-EM0002423]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fgene.2021.644292en
dc.identifier.eissn1664-8021en
dc.identifier.other644292en
dc.identifier.pmid34025716en
dc.identifier.urihttp://hdl.handle.net/10919/106722en
dc.identifier.volume12en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbackground radiationen
dc.subjecttranscriptome analysisen
dc.subjectstress responseen
dc.subjecthomeostatic controlen
dc.subjectde novo transcriptome analysisen
dc.titleDeinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growthen
dc.title.serialFrontiers in Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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