Numerical investigation of flow-through immunoassay in a microchannel

Simple item page
dc.contributorVirginia Tech. Engineering Science and Mechanics Departmenten
dc.contributorJadavpur University. Department of Power Engineeringen
dc.contributor.authorSinha, Ashoken
dc.contributor.authorGanguly, Ranjanen
dc.contributor.authorPuri, Ishwar K.en
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessed2015-04-24en
dc.date.accessioned2015-06-01T16:10:47Zen
dc.date.available2015-06-01T16:10:47Zen
dc.date.issued2010-02-01en
dc.description.abstractImmunomagnetic separation (IMS) is a method to isolate biomaterials from a host fluid in which specifically selected antibodies attached to magnetic particles bind with their corresponding antigens on the surface of the target biological entities. A magnet separates these entities from the fluid through magnetophoresis. The method has promising applications in microscale biosensors. We develop a comprehensive model to characterize the interaction between target species and magnetic particles in microfluidic channels. The mechanics of the separation of target nonmagnetic N particles by magnetic M particles are investigated using a particle dynamics simulation. We consider both interparticle magnetic interactions and the binding of the functionalizing strands of complementary particles. The temporal growth of a particle aggregate and the relative concentrations of M and N particles are investigated under different operating conditions. A particle aggregate first grows and then exhibits periodic washaway about a quasisteady mean size. The washaway frequency and amplitude depend on the initial fractional concentration of N particles while the aggregate size scales linearly with the dipole strength and inversely with the fluid flow rate.en
dc.format.extent7 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSinha, A., Ganguly, R., Puri, I. K. (2010). Numerical investigation of flow-through immunoassay in a microchannel. Journal of Applied Physics, 107(3). doi: 10.1063/1.3284077en
dc.identifier.doihttps://doi.org/10.1063/1.3284077en
dc.identifier.issn0021-8979en
dc.identifier.urihttp://hdl.handle.net/10919/52885en
dc.identifier.urlhttp://scitation.aip.org/content/aip/journal/jap/107/3/10.1063/1.3284077en
dc.language.isoen_USen
dc.publisherAmerican Institute of Physicsen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectIon-mobility spectrometryen
dc.subjectMicrofluidicsen
dc.subjectBiophysical techniquesen
dc.subjectCollision theoriesen
dc.subjectMagnetic fieldsen
dc.titleNumerical investigation of flow-through immunoassay in a microchannelen
dc.title.serialJournal of Applied Physicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2010_Numerical_investigation_of_flow_through.pdf
Size:
903.57 KB
Format:
Adobe Portable Document Format
Download

Collections

Scholarly Works, Biomedical Engineering and Mechanics