Water sample viral contamination detection system

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dc.contributor.assigneeCenter for Innovative Technologyen
dc.contributor.assigneeVirginia Polytechnic Institute and State Universityen
dc.contributor.assigneeVirginia Tech Intellectual Properties, Inc.en
dc.contributor.inventorIjzerman, M. Marianen
dc.contributor.inventorHagedorn, Charles IIIen
dc.contributor.inventorFalkinham, Joseph O. IIIen
dc.date.accessed2016-08-19en
dc.date.accessioned2016-08-24T17:53:47Zen
dc.date.available2016-08-24T17:53:47Zen
dc.date.filed1993-05-25en
dc.date.issued1996-06-18en
dc.description.abstractViral contamination in water samples is detected using a medium which includes both a substrate for .beta.-galactosidase and E. coli with elevated levels of intracellular .beta.-galactosidase. The substrate is chosen to undergo a detectable change (e.g., colorimetric, fluorometric, photometric, etc.) upon cleavage by .beta.-galactosidase. A water sample to be tested for viral contamination is added to the medium. If coliphages are present in the water sample, they will infect, multiply within, and subsequently lyse the E. coli host. Lysis of the E. coli will allow the release of the intracellular .beta.-galactosidase into the media, whereupon the enzyme will cleave the substrate for a detectable reaction. In one particular application, a colorimetric reagent that serves as a substrate for .beta.-galactosidase is dissolved or dispersed within an agar medium, and in another particular application, a colorimetric reagent that serves as a substrate for .beta.-galactosidase is dissolved or dispersed within a liquid medium. Color changes which result from lytic cell infection of the E. coli hosts are easily monitored by researchers or laboratory technicians. The presence of coliphages in a water sample is indicative of the presence of harmful human and animal enteric viruses in the water.en
dc.format.mimetypeapplication/pdfen
dc.identifier.applicationnumber8066865en
dc.identifier.patentnumber5527667en
dc.identifier.urihttp://hdl.handle.net/10919/72318en
dc.identifier.urlhttp://pimg-fpiw.uspto.gov/fdd/67/276/055/0.pdfen
dc.language.isoen_USen
dc.publisherUnited States Patent and Trademark Officeen
dc.subject.cpcC12Q1/34en
dc.subject.cpcC12Q1/54en
dc.subject.cpcG01N2333/924en
dc.subject.uspc435/5en
dc.subject.uspcother435/4en
dc.subject.uspcother435/18en
dc.subject.uspcother435/34en
dc.subject.uspcother435/38en
dc.subject.uspcother435/39en
dc.titleWater sample viral contamination detection systemen
dc.typePatenten
dc.type.dcmitypeTexten
dc.type.patenttypeutilityen

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