Mycoplasma agassizii, an opportunistic pathogen of tortoises, shows very little genetic variation across the Mojave and Sonoran Deserts

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dc.contributor.authorLuzuriaga-Neira, Agustoen
dc.contributor.authorSandmeier, Franziska C.en
dc.contributor.authorWeitzman, Chava L.en
dc.contributor.authorTracy, C. Richarden
dc.contributor.authorBauschlicher, Shalyn N.en
dc.contributor.authorTillett, Richard L.en
dc.contributor.authorAlvarez-Ponce, Daviden
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2021-05-21T15:00:53Zen
dc.date.available2021-05-21T15:00:53Zen
dc.date.issued2021-02-03en
dc.description.abstractMycoplasma agassizii is a common cause of upper respiratory tract disease in Mojave desert tortoises (Gopherus agassizii). So far, only two strains of this bacterium have been sequenced, and very little is known about its patterns of genetic diversity. Understanding genetic variability of this pathogen is essential to implement conservation programs for their threatened, long-lived hosts. We used next generation sequencing to explore the genomic diversity of 86 cultured samples of M. agassizii collected from mostly healthy Mojave and Sonoran desert tortoises in 2011 and 2012. All samples with enough sequencing coverage exhibited a higher similarity to M. agassizii strain PS6(T) (collected in Las Vegas Valley, Nevada) than to strain 723 (collected in Sanibel Island, Florida). All eight genomes with a sequencing coverage over 2x were subjected to multiple analyses to detect single-nucleotide polymorphisms (SNPs). Strikingly, even though we detected 1373 SNPs between strains PS6(T) and 723, we did not detect any SNP between PS6(T) and our eight samples. Our whole genome analyses reveal that M. agassizii strain PS6(T) may be present across a wide geographic extent in healthy Mojave and Sonoran desert tortoises.en
dc.description.notesThis work was supported by grant F19AC00070 from the US Fish and Wildlife Service, and partially supported by Nevada INBRE (funded by grant P20GM103440 from the National Institute of General Medical Sciences, National Institutes of Health). AL and DAP were also supported by a grant from the National Science Foundation (MCB 1818288). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.description.sponsorshipUS Fish and Wildlife ServiceUS Fish & Wildlife Service [F19AC00070]; Nevada INBRE (National Institute of General Medical Sciences, National Institutes of Health) [P20GM103440]; National Science FoundationNational Science Foundation (NSF) [MCB 1818288]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0245895en
dc.identifier.issn1932-6203en
dc.identifier.issue2en
dc.identifier.othere0245895en
dc.identifier.pmid33534823en
dc.identifier.urihttp://hdl.handle.net/10919/103430en
dc.identifier.volume16en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleMycoplasma agassizii, an opportunistic pathogen of tortoises, shows very little genetic variation across the Mojave and Sonoran Desertsen
dc.title.serialPlos Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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