A Network Biology Approach to Denitrification in Pseudomonas aeruginosa

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dc.contributorVirginia Tech. Department of Mathematicsen
dc.contributorVirginia Bioinformatics Instituteen
dc.contributor.authorArat, Sedaen
dc.contributor.authorBullerjahn, George S.en
dc.contributor.authorLaubenbacher, Reinhard C.en
dc.contributor.departmentMathematicsen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2015-10-01T23:25:14Zen
dc.date.available2015-10-01T23:25:14Zen
dc.date.issued2015-02-23en
dc.description.abstractPseudomonas aeruginosa is a metabolically flexible member of the Gammaproteobacteria. Under anaerobic conditions and the presence of nitrate, P. aeruginosa can perform (complete) denitrification, a respiratory process of dissimilatory nitrate reduction to nitrogen gas via nitrite (NO₂), nitric oxide (NO) and nitrous oxide (N₂O). This study focuses on understanding the influence of environmental conditions on bacterial denitrification performance, using a mathematical model of a metabolic network in P. aeruginosa. To our knowledge, this is the first mathematical model of denitrification for this bacterium. Analysis of the long-term behavior of the network under changing concentration levels of oxygen (O₂), nitrate (NO₃), and phosphate (PO₄) suggests that PO₄ concentration strongly affects denitrification performance. The model provides three predictions on denitrification activity of P. aeruginosa under various environmental conditions, and these predictions are either experimentally validated or supported by pertinent biological literature. One motivation for this study is to capture the effect of PO₄ on a denitrification metabolic network of P. aeruginosa in order to shed light on mechanisms for greenhouse gas N₂O accumulation during seasonal oxygen depletion in aquatic environments such as Lake Erie (Laurentian Great Lakes, USA). Simulating the microbial production of greenhouse gases in anaerobic aquatic systems such as Lake Erie allows a deeper understanding of the contributing environmental effects that will inform studies on, and remediation strategies for, other hypoxic sites worldwide.en
dc.description.sponsorshipU.S. Department of Energyen
dc.description.sponsorshipOffice of Scienceen
dc.description.sponsorshipNational Science Foundationen
dc.description.sponsorshipDirectorate for Geosciencesen
dc.description.sponsorshipDE-AC02-05CH11231en
dc.description.sponsorshipOCE-0927277en
dc.format.extent12 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationArat, S., Bullerjahn, G. S., & Laubenbacher, R. (2015). A Network Biology Approach to Denitrification in Pseudomonas Aeruginosa. Plos One, 10(2), e0118235. doi: 10.1371/journal.pone.0118235en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0118235en
dc.identifier.issn1932-6203en
dc.identifier.issue2en
dc.identifier.urihttp://hdl.handle.net/10919/56683en
dc.identifier.volume10en
dc.language.isoen_USen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAlgebraen
dc.subjectBacteriaen
dc.subjectDiscrete modelsen
dc.subjectDissolved substancesen
dc.subjectEscherichia-colien
dc.subjectGeneen
dc.subjectLake erieen
dc.subjectNitrous-oxide reductaseen
dc.subjectPhosphateen
dc.subjectPhosphorusen
dc.titleA Network Biology Approach to Denitrification in Pseudomonas aeruginosaen
dc.title.serialPloS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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