Diverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus
| dc.contributor.author | Sabbagh, Ubadah | en |
| dc.contributor.author | Govindaiah, Gubbi | en |
| dc.contributor.author | Somaiya, Rachana D. | en |
| dc.contributor.author | Ha, Ryan V. | en |
| dc.contributor.author | Wei, Jessica C. | en |
| dc.contributor.author | Guido, William | en |
| dc.contributor.author | Fox, Michael A. | en |
| dc.contributor.department | Biological Sciences | en |
| dc.contributor.department | Fralin Biomedical Research Institute | en |
| dc.contributor.department | School of Neuroscience | en |
| dc.contributor.department | Virginia Tech Carilion School of Medicine | en |
| dc.date.accessioned | 2020-06-25T13:47:15Z | en |
| dc.date.available | 2020-06-25T13:47:15Z | en |
| dc.date.issued | 2020-05-19 | en |
| dc.description.abstract | In the visual system, retinal axons convey visual information from the outside world to dozens of distinct retinorecipient brain regions and organize that information at several levels, including either at the level of retinal afferents, cytoarchitecture of intrinsic retinorecipient neurons, or a combination of the two. Two major retinorecipient nuclei which are densely innervated by retinal axons are the dorsal lateral geniculate nucleus, which is important for classical image-forming vision, and ventral LGN (vLGN), which is associated with non-image-forming vision. The neurochemistry, cytoarchitecture, and retinothalamic connectivity in vLGN remain unresolved, raising fundamental questions of how it receives and processes visual information. To shed light on these important questions, used in situ hybridization, immunohistochemistry, and genetic reporter lines to identify and characterize novel neuronal cell types in mouse vLGN. Not only were a high percentage of these cells GABAergic, we discovered transcriptomically distinct GABAergic cell types reside in the two major laminae of vLGN, the retinorecipient, external vLGN (vLGNe) and the non-retinorecipient, internal vLGN (vLGNi). Furthermore, within vLGNe, we identified transcriptionally distinct subtypes of GABAergic cells that are distributed into four adjacent sublaminae. Using trans-synaptic viral tracing and in vitro electrophysiology, we found cells in each these vLGNe sublaminae receive monosynaptic inputs from retina. These results not only identify novel subtypes of GABAergic cells in vLGN, they suggest the subtype-specific laminar distribution of retinorecipient cells in vLGNe may be important for receiving, processing, and transmitting light-derived signals in parallel channels of the subcortical visual system. | en |
| dc.description.sponsorship | National Eye Institute, Grant/Award Number: EY012716, EY021222 and EY030568 | en |
| dc.description.sponsorship | National Institute of Neurological Disorders and Stroke, Grant/ Award Number: NS105141 and NS113459 | en |
| dc.description.sponsorship | National Institutes of Health, Grant/Award Number: EY021222, EY030568, NS105141, NS113459 and EY012716 | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1111/jnc.15101 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/99109 | en |
| dc.language.iso | en | en |
| dc.publisher | Wiley | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | circuit | en |
| dc.subject | GABAergic | en |
| dc.subject | geniculate | en |
| dc.subject | retina | en |
| dc.subject | thalamus | en |
| dc.subject | visual | en |
| dc.title | Diverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus | en |
| dc.title.serial | Journal of Neurochemistry | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |