Diverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleus

Simple item page
dc.contributor.authorSabbagh, Ubadahen
dc.contributor.authorGovindaiah, Gubbien
dc.contributor.authorSomaiya, Rachana D.en
dc.contributor.authorHa, Ryan V.en
dc.contributor.authorWei, Jessica C.en
dc.contributor.authorGuido, Williamen
dc.contributor.authorFox, Michael A.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentFralin Biomedical Research Instituteen
dc.contributor.departmentSchool of Neuroscienceen
dc.contributor.departmentVirginia Tech Carilion School of Medicineen
dc.date.accessioned2020-06-25T13:47:15Zen
dc.date.available2020-06-25T13:47:15Zen
dc.date.issued2020-05-19en
dc.description.abstractIn the visual system, retinal axons convey visual information from the outside world to dozens of distinct retinorecipient brain regions and organize that information at several levels, including either at the level of retinal afferents, cytoarchitecture of intrinsic retinorecipient neurons, or a combination of the two. Two major retinorecipient nuclei which are densely innervated by retinal axons are the dorsal lateral geniculate nucleus, which is important for classical image-forming vision, and ventral LGN (vLGN), which is associated with non-image-forming vision. The neurochemistry, cytoarchitecture, and retinothalamic connectivity in vLGN remain unresolved, raising fundamental questions of how it receives and processes visual information. To shed light on these important questions, used in situ hybridization, immunohistochemistry, and genetic reporter lines to identify and characterize novel neuronal cell types in mouse vLGN. Not only were a high percentage of these cells GABAergic, we discovered transcriptomically distinct GABAergic cell types reside in the two major laminae of vLGN, the retinorecipient, external vLGN (vLGNe) and the non-retinorecipient, internal vLGN (vLGNi). Furthermore, within vLGNe, we identified transcriptionally distinct subtypes of GABAergic cells that are distributed into four adjacent sublaminae. Using trans-synaptic viral tracing and in vitro electrophysiology, we found cells in each these vLGNe sublaminae receive monosynaptic inputs from retina. These results not only identify novel subtypes of GABAergic cells in vLGN, they suggest the subtype-specific laminar distribution of retinorecipient cells in vLGNe may be important for receiving, processing, and transmitting light-derived signals in parallel channels of the subcortical visual system.en
dc.description.sponsorshipNational Eye Institute, Grant/Award Number: EY012716, EY021222 and EY030568en
dc.description.sponsorshipNational Institute of Neurological Disorders and Stroke, Grant/ Award Number: NS105141 and NS113459en
dc.description.sponsorshipNational Institutes of Health, Grant/Award Number: EY021222, EY030568, NS105141, NS113459 and EY012716en
dc.identifier.doihttps://doi.org/10.1111/jnc.15101en
dc.identifier.urihttp://hdl.handle.net/10919/99109en
dc.language.isoen_USen
dc.publisherWileyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcircuiten
dc.subjectGABAergicen
dc.subjectgeniculateen
dc.subjectretinaen
dc.subjectthalamusen
dc.subjectvisualen
dc.titleDiverse GABAergic neurons organize into subtype-specific sublaminae in the ventral lateral geniculate nucleusen
dc.title.serialJournal of Neurochemistryen
dc.typeArticle - Refereeden

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jnc.15101.pdf
Size:
5.95 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Scholarly Works, Fralin Biomedical Research Institute at VTC
Scholarly Works, Biological Sciences
Scholarly Works, School of Neuroscience
Scholarly Works, Virginia Tech Carilion School of Medicine (VTCSOM)